RT Journal Article SR Electronic(1) A1 Oelschlägel, Michel A1 Rückert, Christian A1 Kalinowski, Jörn A1 Schmidt, Gert A1 Schlömann, Michael A1 Tischler, DirkYR 2015 T1 Sphingopyxis fribergensis sp. nov., a soil bacterium with the ability to degrade styrene and phenylacetic acid JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_9 SP 3008 OP 3015 DO https://doi.org/10.1099/ijs.0.000371 PB Microbiology Society, SN 1466-5034, AB Strain Kp5.2T is an aerobic, Gram-negative soil bacterium that was isolated in Freiberg, Saxony, Germany. The cells were motile and rod-shaped. Optimal growth was observed at 20–30 °C. The fatty acids of strain Kp5.2T comprised mainly C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH). The major respiratory quinone was Q-10. The major polar lipids of strain Kp5.2T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.7 %. Sequencing of the 16S rRNA gene of strain Kp5.2T allowed its classification into the family Sphingomonadaceae, and the sequence showed the highest similarity to those of members of the genus Sphingopyxis, with Sphingopyxis italica SC13E-S71T (99.15 % similarity), Sphingopyxis panaciterrae Gsoil 124T (98.96 %), Sphingopyxis chilensis S37T (98.90 %) and Sphingopyxis bauzanensis BZ30T (98.51 %) as the nearest neighbours. DNA–DNA hybridization and further characterization revealed that strain Kp5.2T can be considered to represent a novel species of the genus Sphingopyxis. Hence, the name Sphingopyxis fribergensis sp. nov. is proposed, with the type strain Kp5.2T ( = DSM 28731T = LMG 28478T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000371