%0 Journal Article %A Rashid, M. Harun-or %A Young, J. Peter W. %A Everall, Isobel %A Clercx, Pia %A Willems, Anne %A Santhosh Braun, Markus %A Wink, Michael %T Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_9 %P 3037-3045 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.000373 %I Microbiology Society, %X Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnII) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA–DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA–DNA hybridization showed 50–62 % relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoli) and 50–60 % relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87–92 % with their close relatives and 88–89 % with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27T = LMG 28441T = DSM 29286T), Rhizobium bangladeshense sp. nov. (type strain BLR175T = LMG 28442T = DSM 29287T) and Rhizobium binae sp. nov. (type strain BLR195T = LMG 28443T = DSM 29288T), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000373