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Volume 59, Issue 6

sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake Free

Abstract

A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1, was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5–13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus , showing highest similarity to JCM 13285 (98.0 %). However, DNA–DNA hybridization indicated low levels of genomic relatedness with JCM 13285 (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C (38.6 %) and anteiso-C (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1 (=KCTC 13181=CGMCC 1.6854) should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): AL, aminolipid , APL, aminophospholipid , DPG, diphosphatidylglycerol , PE, phosphatidylethanolamine and PG, phosphatidylglycerol
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2009-06-01
2024-04-20
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Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake
Int J Syst Evol Microbiol 59, 1460 (2009); https://doi.org/10.1099/ijs.0.000653-0
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vol. , part 6, pp. 1460 - 1464

Two-dimensional TLC of the polar lipids of strain YC1 and JCM 13285 .

Transmission electron micrograph of cells of strain YC1 grown on LB agar for 24 h at 30°C.

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