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Abstract
A taxonomic study was carried out on strain A-11-3T, which was isolated from an oil-enriched consortia from the surface seawater of Hong-Deng dock in the Straits of Malacca and Singapore. Cells were aerobic, Gram-negative, non-spore-forming irregular rods. The strain was catalase- and oxidase-negative. It grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16S rRNA gene sequence comparisons showed that strain A-11-3T was most closely related to the type strains of Alcanivorax jadensis (96.8 % sequence similarity), Alcanivorax borkumensis (96.8 %), Alcanivorax dieselolei (94.8 %), Alcanivorax venustensis (94.2 %) and Alcanivorax balearicus (94.0 %). The predominant fatty acids were C16 : 0 (31.2 %), C18 : 1 ω7c (24.8 %), C18 : 0 (9.6 %), C12 : 0 (8.3 %), C16 : 1 ω7c (8.3 %) and C16 : 0 3-OH (5.1 %). The G+C content of the genomic DNA was 54.7 mol%. Moreover, the strain produced lipopeptides as its surface-active compounds. According to physiological and biochemical tests, DNA–DNA hybridization results and sequence comparisons of the 16S–23S internal transcribed spacer, the gyrB gene and the alkane hydroxylase gene alkB1, strain A-11-3T was affiliated with the genus Alcanivorax but could be readily distinguished from recognized Alcanivorax species. Therefore strain A-11-3T represents a novel species of the genus Alcanivorax for which the name Alcanivorax hongdengensis sp. nov. is proposed. The type strain is A-11-3T (=CGMCC 1.7084T=LMG 24624T=MCCC 1A01496T).
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