Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field Mochimaru, Hanako and Tamaki, Hideyuki and Hanada, Satoshi and Imachi, Hiroyuki and Nakamura, Kohei and Sakata, Susumu and Kamagata, Yoichi,, 59, 714-718 (2009), doi = https://doi.org/10.1099/ijs.0.001677-0, publicationName = Microbiology Society, issn = 1466-5026, abstract= A mesophilic, methylotrophic methanogen, strain MobMT, was isolated from a natural gas field in Japan. Strain MobMT grew on methanol and methylamines, but not on H2/CO2, formate, acetate or dimethyl sulfide. The cells were motile, irregular cocci (diameter, 0.9–1.2 μm) and occurred singly, in pairs, as tetracocci or (occasionally) as aggregates. Strain MobMT grew at 9–37 °C (optimally at 30 °C) and at pH 6.1–7.8 (optimally at pH 6.5). Sodium and magnesium were required for growth, at 0.1–1.0 M Na+ (optimally at 0.35 M) and 10–400 mM Mg2+ (optimally at 15–25 mM). The G+C content of the genomic DNA was 42.4 mol%. 16S rRNA gene sequencing revealed that the isolate is a member of the genus Methanolobus, but distinct from its closest neighbours, Methanolobus tindarius DSM 2278T (sequence similarity, 98.0 %) and Methanolobus vulcani DSM 3029T (98.1 %). On the basis of phenotypic and phylogenetic features of MobMT, it is clear that this strain represents a novel species of the genus Methanolobus, for which the name Methanolobus profundi sp. nov. is proposed. The type strain is MobMT (=DSM 21213T=NBRC 104158T)., language=, type=