RT Journal Article SR Electronic(1) A1 Mochimaru, Hanako A1 Tamaki, Hideyuki A1 Hanada, Satoshi A1 Imachi, Hiroyuki A1 Nakamura, Kohei A1 Sakata, Susumu A1 Kamagata, YoichiYR 2009 T1 Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 4 SP 714 OP 718 DO https://doi.org/10.1099/ijs.0.001677-0 PB Microbiology Society, SN 1466-5034, AB A mesophilic, methylotrophic methanogen, strain MobMT, was isolated from a natural gas field in Japan. Strain MobMT grew on methanol and methylamines, but not on H2/CO2, formate, acetate or dimethyl sulfide. The cells were motile, irregular cocci (diameter, 0.9–1.2 μm) and occurred singly, in pairs, as tetracocci or (occasionally) as aggregates. Strain MobMT grew at 9–37 °C (optimally at 30 °C) and at pH 6.1–7.8 (optimally at pH 6.5). Sodium and magnesium were required for growth, at 0.1–1.0 M Na+ (optimally at 0.35 M) and 10–400 mM Mg2+ (optimally at 15–25 mM). The G+C content of the genomic DNA was 42.4 mol%. 16S rRNA gene sequencing revealed that the isolate is a member of the genus Methanolobus, but distinct from its closest neighbours, Methanolobus tindarius DSM 2278T (sequence similarity, 98.0 %) and Methanolobus vulcani DSM 3029T (98.1 %). On the basis of phenotypic and phylogenetic features of MobMT, it is clear that this strain represents a novel species of the genus Methanolobus, for which the name Methanolobus profundi sp. nov. is proposed. The type strain is MobMT (=DSM 21213T=NBRC 104158T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001677-0