1887

Abstract

An aerobic, pink-pigmented, budding and rosette-forming bacterium was isolated from an acidic peat bog and designated strain A10. The 16S rRNA gene sequence analysis showed that strain A10 was a member of the order and belonged to a phylogenetic lineage defined by the genus , with 90 % sequence similarity to that of , the only taxonomically described organism of this group. Ellipsoid-shaped cells of strain A10 were uniformly covered with crateriform pits and possessed long (up to 10–15 μm) and unusually thick (0.5–0.7 μm) stalks of a unique ultrastructure. Thin sections revealed a complex intracellular membrane system compartmentalizing the cells. Strain A10 was a moderately acidophilic, mesophilic organism capable of growth at pH values between 3.8 and 7.2 (with an optimum at pH 5.5–6.0) and at temperatures between 10 and 30 °C (with an optimum at 20–25 °C). The major fatty acids were C, C 5 and C 5 and the major quinone was MK-6. Cells of strain A10 contained high amounts of bound saturated and monounsaturated C–C (-1) hydroxy fatty acids. The G+C content of the DNA was 62.5 mol%. The unique cell morphology, the capability of growth in acidic conditions and a number of chemotaxonomic and genotypic characteristics served to differentiate strain A10 from . Based on these data, the novel isolate should be considered as representing a novel genus and species of planctomycetes, for which the name gen. nov., sp. nov. is proposed The type strain is A10 (=DSM 19928=VKM B-2478).

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2009-02-01
2024-03-28
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References

  1. Bauld, J. & Staley, J. T.(1976).Planctomyces maris sp. nov.: a marine isolate of the Planctomyces-Blastocaulis group of budding bacteria. J Gen Microbiol 97, 45–55.[CrossRef] [Google Scholar]
  2. Dedysh, S. N., Pankratov, T. A., Belova, S. E., Kulichevskaya, I. S. & Liesack, W.(2006). Phylogenetic analysis and in situ identification of Bacteria community composition in an acidic Sphagnum peat bog. Appl Environ Microbiol 72, 2110–2117.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1989).phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166. [Google Scholar]
  4. Franzmann, P. D. & Skerman, V. B. D.(1984).Gemmata obscuriglobus, a new genus and species of the budding bacteria. Antonie van Leeuwenhoek 50, 261–268.[CrossRef] [Google Scholar]
  5. Fuerst, J. A.(2005). Intracellular compartmentation in planctomycetes. Annu Rev Microbiol 59, 299–328.[CrossRef] [Google Scholar]
  6. Goossens, H., de Leeuw, J. W., Rijpstra, W. I. C., Meyburg, G. J. & Schenck, P. A.(1989). Lipids and their mode of occurrence in bacteria and sediments. I. A methodological study of the lipid composition of Acintobacter calcoaceticus LMD 79–41. Org Geochem 14, 15–25.[CrossRef] [Google Scholar]
  7. Hirsch, P. & Müller, M.(1985).Planctomyces limnophilus sp. nov., a stalked and budding bacterium from freshwater. Syst Appl Microbiol 6, 276–280.[CrossRef] [Google Scholar]
  8. Hirsch, P., Müller, M. & Schlesner, H.(1977). New aquatic budding and prosthecate bacteria and their taxonomic position. In Aquatic Microbiology, pp. 107–133. Soc. Appl. Bact. Sympos. Ser. 6. Edited by Skinner & Shewan. London, UK: Academic Press.
  9. Ivanova, A. O. & Dedysh, S. N.(2006). High abundance of Planctomycetes in anoxic layers of a Sphagnum peat bog. Microbiology 75, 716–719.[CrossRef] [Google Scholar]
  10. Kulichevskaya, I. S., Pankratov, T. A. & Dedysh, S. N.(2006). Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation approaches. Microbiology (English translation of Mikrobiologiia) 75, 329–335.[CrossRef] [Google Scholar]
  11. Kulichevskaya, I. S., Ivanova, A. O., Belova, S. E., Baulina, O. I., Bodelier, P. L. E., Rijpstra, W. I. C., Sinninghe Damsté, J. S., Zavarzin, G. A. & Dedysh, S. N.(2007).Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands. Int J Syst Evol Microbiol 57, 2680–2687.[CrossRef] [Google Scholar]
  12. Kulichevskaya, I. S., Ivanova, A. O., Baulina, O. I., Bodelier, P. L. E., Sinninghe Damsté, J. S. & Dedysh, S. N.(2008).Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands. Int J Syst Evol Microbiol 58, 1186–1193.[CrossRef] [Google Scholar]
  13. Lindsay, M. R., Webb, R. I., Strous, M., Jetten, M. S., Butler, M. K., Forde, R. J. & Fuerst, J. A.(2001). Cell compartmentalization in Planctomycetes: novel types of structural organization for the bacterial cell. Arch Microbiol 175, 413–429.[CrossRef] [Google Scholar]
  14. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S., Jobb, G. & other authors(2004). ARB: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  15. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  16. Owen, R. J., Lapage, S. P. & Hill, L. R.(1969). Determination of base composition from melting profiles in dilute buffers. Biopolymers 7, 503–516.[CrossRef] [Google Scholar]
  17. Pearson, A., Budin, M. & Brocks, J. J.(2003). Phylogenetic and biochemical evidence for sterol synthesis in the bacterium Gemmata obscuriglobus. Proc Natl Acad Sci U S A 100, 15352–15357.[CrossRef] [Google Scholar]
  18. Schlesner, H.(1989).Planctomyces brasiliensis sp. nov., a halotolerant bacterium from a salt pit. Syst Appl Microbiol 12, 159–161.[CrossRef] [Google Scholar]
  19. Sittig, M. & Schlesner, H.(1993). Chemotaxonomic investigation of various prosthecate and/or budding bacteria. Syst Appl Microbiol 16, 92–103.[CrossRef] [Google Scholar]
  20. Staley, J. T., Fuerst, J. A., Giovannoni, S. & Schlesner, H.(1992). The order Planctomycetales and the genera Planctomyces, Pirellula, Gemmata and Isosphaera. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3710–3731. Edited by A. Balows, H. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. NewYork: Springer.
  21. Starr, M. R. & Schmidt, J. M.(1984).Planctomyces stranskae (ex Wawrik 1952) sp. nov., nom. rev. and Planctomyces guttaeformis (ex Hortobágyi 1965) sp. nov., nom. rev. Int J Syst Bacteriol 34, 470–477.[CrossRef] [Google Scholar]
  22. Wang, J., Jenkins, C., Webb, R. I. & Fuerst, J. A.(2002). Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater. Appl Environ Microbiol 68, 417–422.[CrossRef] [Google Scholar]
  23. Ward, N., Staley, J. T., Fuerst, J. A., Giovannoni, S., Schlesner, H. & Stackebrandt, E.(2006). The order Planctomycetales, including the genera Planctomyces, Pirellula, Gemmata and Isosphaera and the Candidatus genera Brocadia, Kuenenia and Scalindua. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 757–793. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
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Comparison of the phospholipid fatty acid compositions of strain A10 ( gen. nov., sp. nov.) and DSM 5831 . [PDF](77 KB)

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Electron micrographs of negatively stained preparations of cells of strain A10 . [PDF](54 KB)

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