1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 5

sp. nov., isolated from stream sediment Free

Abstract

A Gram-negative, strictly aerobic, non-spore-forming, rod-shaped, red–pink bacterium, designated strain Dae14, was isolated from stream sediment collected near Daecheong Dam, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that strain Dae14 belonged to the genus . Sequence similarities between strain Dae14 and the type strains of species with validly published names ranged from 91.3 to 94.3 %. The predominant cellular fatty acids of strain Dae14 were iso-C, C 5, summed feature 5 (iso-C I and/or anteiso-C B) and iso-C. The DNA G+C content was 62.2 mol%. Results of phylogenetic, chemotaxonomic and phenotypic characterization indicated that strain Dae14 can be distinguished from all known species and represents a novel species, for which the name sp. nov. is proposed, with Dae14 (=KCTC 22258=LMG 24498) as the type strain.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002444-0
2009-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/1183.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002444-0&mimeType=html&fmt=ahah

References

  1. Atlas, R. M.(1993).Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  2. Baik, K. S., Seong, C. N., Moon, E. Y., Park, Y.-D., Yi, H. & Chun, J.(2006).Hymenobacter rigui sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 56, 2189–2192.[CrossRef] [Google Scholar]
  3. Benson, D. A., Karsch-Mizrachi, I., Lipman, D. J., Ostell, J. & Wheeler, D. L.(2005). GenBank. Nucleic Acids Res 33, D34–D38.[CrossRef] [Google Scholar]
  4. Buck, J. D.(1982). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  5. Buczolits, S., Denner, E. B. M., Vybiral, D., Wieser, M., Kämpfer, P. & Busse, H. J.(2002). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52, 445–456. [Google Scholar]
  6. Buczolits, S., Denner, E. B. M., Kämpfer, P. & Busse, H. J.(2006). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. Int J Syst Evol Microbiol 56, 2071–2078.[CrossRef] [Google Scholar]
  7. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam, S. A., McGarrell, D. M., Garrity, G. M. & Tiedje, J. M.(2005). The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33, D294–D296. [Google Scholar]
  8. Collins, M. D., Hutson, R. A., Grant, I. R. & Patterson, M. F.(2000). Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. Int J Syst Evol Microbiol 50, 731–734.[CrossRef] [Google Scholar]
  9. Fautz, E. & Reichenbach, H.(1980). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8, 87–91.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  12. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  13. Hiraishi, A., Ueda, Y., Ishihara, J. & Mori, T.(1996). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42, 457–469.[CrossRef] [Google Scholar]
  14. Hirsch, P., Ludwig, W., Hethke, C., Sittig, M., Hoffmann, B. & Gallikowski, C. A.(1998).Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Syst Appl Microbiol 21, 374–383.[CrossRef] [Google Scholar]
  15. Im, W.-T., Kang, M. S., Park, H. Y., Kim, M.-K. & Lee, S.-T.(2003). Culturable bacterial strain's diversity of environmental samples. In Proceedings of the International Meeting of the Federation of Korean Microbiological Societies, abstract B4023, p. 165. Seoul: Federation of Korean Microbiological Societies.
  16. Kim, M.-K., Im, W.-T. & Lee, S.-T.(2008).Hymenobacter soli sp. nov., isolated from grass soil. Int J Syst Evol Microbiol 58, 211–213. [Google Scholar]
  17. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  18. Kouker, G. & Jaeger, K.-E.(1987). Specific and sensitive plate assay for bacterial lipases. Appl Environ Microbiol 53, 211–213. [Google Scholar]
  19. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  20. Mesbah, M. & Whitman, W. B.(1989). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef] [Google Scholar]
  21. Moore, D. D. & Dowhan, D.(1995). Preparation and analysis of DNA. In Current Protocols in Molecular Biology, pp. 2–11. Edited by F. W. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fattyacids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Ten, L. N., Im, W.-T., Kim, M.-K., Kang, M.-S. & Lee, S.-T.(2004). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56, 375–382.[CrossRef] [Google Scholar]
  25. Ten, L. N., Jung, H.-M., Yoo, S.-A., Im, W.-T. & Lee, S.-T.(2008).Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea. J Microbiol 46, 519–524.[CrossRef] [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Tschech, A. & Pfennig, N.(1984). Growth yield increase linked to caffeate reduction in Acetobacterium woodii. Arch Microbiol 137, 163–167.[CrossRef] [Google Scholar]
  28. Widdel, F. & Bak, F.(1992). Gram-negative mesophilic sulfate reducing bacteria. In The Prokaryotes, 2nd edn, pp. 3352–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer–Verlag.
  29. Widdel, F., Kohring, G.-W. & Mayer, F.(1983). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov. sp. nov., and Desulfonema magnum sp. nov. Arch Microbiol 134, 286–294.[CrossRef] [Google Scholar]
  30. Zhang, Q., Liu, C., Tang, Y., Zhou, G., Shen, P., Fang, C. & Yokota, A.(2007).Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 57, 1752–1756.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002444-0
Loading
Hymenobacter daecheongensis sp. nov., isolated from stream sediment
Int J Syst Evol Microbiol 59, 1183 (2009); https://doi.org/10.1099/ijs.0.002444-0
/content/journal/ijsem/10.1099/ijs.0.002444-0
/content/journal/ijsem/10.1099/ijs.0.002444-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1183 - 1187

Cellular fatty acid profiles of Dae14 and related strains. [PDF](53 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error