1887

Abstract

An aerobic, Gram-negative, ovoid to rod-shaped bacterial isolate, strain 81-2, was isolated from deep-sea sediment of the Western Pacific Ocean. Strain 81-2 was motile, formed faint pink colonies, and was catalase-positive, weakly positive for oxidase and required NaCl for growth. It did not synthesize bacteriochlorophyll and its DNA G+C content was 62.3 mol%. The 16S rRNA gene sequence of strain 81-2 indicated that it was a member of the clade of the class , with moderate bootstrap support for inclusion in the genus . Its closest phylogenetic neighbour was the type strain of , which shared 95.8 % 16S rRNA gene sequence similarity; strain 81-2 was <95.0 % similar to strains of other related species and genera. Phenotypic, chemotaxonomic and phylogenetic data support assignment of this strain to the genus as a representative of a novel species. The name sp. nov. is proposed, with strain 81-2 (=MCCC 1A00293=CGMCC 1.7083=LMG 24575) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002477-0
2009-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/1116.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002477-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors)(1995).Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
  2. Biebl, H., Allgaier, M., Lünsdorf, H., Pukall, R., Tindall, B. J. & Wagner-Döbler, I.(2005).Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a. Int J Syst Evol Microbiol 55, 2377–2383.[CrossRef] [Google Scholar]
  3. Boettcher, K. J., Barber, B. J. & Singer, J. T.(1999). Use of antibacterial agents to elucidate the etiology of juvenile oyster disease (JOD) in Crassostrea virginica and numerical dominance of an α-proteobacterium in JOD-affected animals. Appl Environ Microbiol 65, 2534–2539. [Google Scholar]
  4. Boettcher, K. J., Geaghan, K. K., Maloy, A. P. & Barber, B. J.(2005).Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55, 1531–1537.[CrossRef] [Google Scholar]
  5. Buchan, A., González, J. M. & Moran, M. A.(2005). Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71, 5665–5677.[CrossRef] [Google Scholar]
  6. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. González, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B.(1997).Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int J Syst Bacteriol 47, 773–780.[CrossRef] [Google Scholar]
  8. González, J. M., Covert, J. S., Whitman, W. B., Henriksen, J. R., Mayer, F., Scharf, B., Schmitt, R., Buchan, A., Fuhrman, J. A. & other authors(2003).Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53, 1261–1269.[CrossRef] [Google Scholar]
  9. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, N. & other authors(1998). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef] [Google Scholar]
  10. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Braker, G. & Hirsch, P.(1998).Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48, 1363–1372.[CrossRef] [Google Scholar]
  11. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P.(1999).Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef] [Google Scholar]
  12. Lafay, B., Ruimy, R., Rausch de Traubenberg, C., Breittmayer, V., Gauthier, M. J. & Christen, R.(1995).Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 45, 290–296.[CrossRef] [Google Scholar]
  13. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  14. Martens, T., Heidorn, T., Pukall, R., Simon, M., Tindall, B. J. & Brinkhoff, T.(2006). Reclassification of Roseobactergallaeciensis Ruiz-Ponte et al. 1998 as Phaeobactergallaeciensis gen. nov., comb. nov., description of Phaeobacterinhibens sp. nov., reclassification of Ruegeriaalgicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovumalgicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56, 1293–1304.[CrossRef] [Google Scholar]
  15. Martínez-Cánovas, M. J., Quesada, E., Martínez-Checa, F., del Moral, A. & Béjar, V.(2004).Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria. Int J Syst Evol Microbiol 54, 1735–1740.[CrossRef] [Google Scholar]
  16. Mesbah, M. & Whitman, W. B.(1989). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef] [Google Scholar]
  17. Mrozik, A., Piotrowska-Seget, Z. & Labuzek, S.(2004). Changes in whole cell-derived fatty acids induced by naphthalene in bacteria from genus Pseudomonas. Microbiol Res 159, 87–95.[CrossRef] [Google Scholar]
  18. Pukall, R., Buntefuss, D., Frühling, A., Rohde, M., Kroppenstedt, R. M., Burghardt, J., Lebaron, P., Bernard, L. & Stackebrandt, E.(1999).Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria. Int J Syst Bacteriol 49, 513–519.[CrossRef] [Google Scholar]
  19. Ruiz-Ponte, C., Cilia, V., Lambert, C. & Nicolas, J. L.(1998).Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus. Int J Syst Bacteriol 48, 537–542.[CrossRef] [Google Scholar]
  20. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Schaefer, J. K., Goodwin, K. D., McDonald, I. R., Murrell, J. C. & Oremland, R. S.(2002).Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. Int J Syst Evol Microbiol 52, 851–859.[CrossRef] [Google Scholar]
  22. Shiba, T.(1991).Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14, 140–145.[CrossRef] [Google Scholar]
  23. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002477-0
Loading
/content/journal/ijsem/10.1099/ijs.0.002477-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1116-1121

Plasmid profile observed in the strain 81-2 . A λ DNA/ dIII marker was used as the size reference (lane M), and plasmid DNA is indicated by the arrow (lane 1).



IMAGE
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error