1887

Abstract

Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus . These strains were most closely related to ATCC 25611, with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were and (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of JCM 12248 and JCM 12250. The isolates could be differentiated from JCM 11140 on the basis of mannose fermentation and -fucosidase activity. The isolates could not be distinguished from or using conventional biochemical tests. DNA–DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to JCM 11140, JCM 12248 and JCM 12250. On the basis of these data, strains 04013, 04021, 04043, 04052, 0406, 04113, 04111 and 04161 represent a novel species, for which the name sp. nov. is proposed. The type strain is 04052 (=JCM 15124 =CCUG 56137).

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2009-02-01
2024-04-16
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References

  1. Baumgartner, J. C., Bae, K. S., Xia, T., Whitt, J. & David, L. L.(1999). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and polymerase chain reaction for differentiation of Prevotella intermedia and Prevotella nigrescens. J Endod 25, 324–328.[CrossRef] [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Holdeman, L. V., Cato, E. P. & Moore, W. E. C.(1977).Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  5. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  6. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E.(1988). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef] [Google Scholar]
  7. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A. & other authors(2007).clustalw and clustalx version 2.0. Bioinformatics 23, 2947–2948.[CrossRef] [Google Scholar]
  8. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  9. Miller, L. T.(1982). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  10. Saito, H. & Miura, K.(1963). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef] [Google Scholar]
  11. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  12. Sakamoto, M., Suzuki, M., Umeda, M., Ishikawa, I. & Benno, Y.(2002). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52, 841–849.[CrossRef] [Google Scholar]
  13. Sakamoto, M., Suzuki, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y.(2004).Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 54, 877–883.[CrossRef] [Google Scholar]
  14. Sakamoto, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y.(2005a).Prevotella multiformis sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 815–819.[CrossRef] [Google Scholar]
  15. Sakamoto, M., Umeda, M., Ishikawa, I. & Benno, Y.(2005b).Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 1839–1843.[CrossRef] [Google Scholar]
  16. Shah, H. N.(1992). The genus Bacteroides and related taxa. In The Prokaryotes, 2nd edn, pp. 3593–3607. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  17. Shah, H. N. & Gharbia, S. E.(1992). Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int J Syst Bacteriol 42, 542–546.[CrossRef] [Google Scholar]
  18. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
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vol. , part 2, pp. 319 - 322

Sequence of the primer pair for and alignment of the 16S rRNA gene sequences of related species. [PDF](464 KB)



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