RT Journal Article SR Electronic(1) A1 Sakamoto, Mitsuo A1 Kumada, Hidefumi A1 Hamada, Nobushiro A1 Takahashi, Yusuke A1 Okamoto, Masaaki A1 Bakir, Mohammad Abdul A1 Benno, YoshimiYR 2009 T1 Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 2 SP 319 OP 322 DO https://doi.org/10.1099/ijs.0.002626-0 PB Microbiology Society, SN 1466-5034, AB Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus Prevotella. These strains were most closely related to Prevotella intermedia ATCC 25611T, with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were Prevotella pallens and Prevotella nigrescens (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of P. intermedia JCM 12248T and P. nigrescens JCM 12250T. The isolates could be differentiated from P. pallens JCM 11140T on the basis of mannose fermentation and α-fucosidase activity. The isolates could not be distinguished from P. intermedia or P. nigrescens using conventional biochemical tests. DNA–DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to P. pallens JCM 11140T, P. intermedia JCM 12248T and P. nigrescens JCM 12250T. On the basis of these data, strains 04013, 04021, 04043, 04052T, 0406, 04113, 04111 and 04161 represent a novel Prevotella species, for which the name Prevotella falsenii sp. nov. is proposed. The type strain is 04052T (=JCM 15124T =CCUG 56137T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.002626-0