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Volume 59, Issue 8

sp. nov., a moderately halophilic bacterium isolated from a salt lake in China Free

Abstract

A Gram-positive, moderately halophilic, alkalitolerant, strictly aerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny3, was isolated from a sediment sample collected from a salt lake in the Qaidam Basin of north-west China. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Growth occurred with 1–20 % (w/v) total salts (optimum, 5–10 %) and at pH 6.0–10.5 (optimum, pH 7.5–8.0) and 10–55 °C (optimum, 35–40 °C). It was unable to grow with NaCl as the only salt. -Diaminopimelic acid was present in the cell-wall peptidoglycan. The strain contained menaquinone 7 (MK-7) as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as polar lipids. The major cellular fatty acids were anteiso-C and anteiso-C. The DNA G+C content was 40.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM kkny3 belonged to the genus , and was most closely related to the type strains of (97.1 % similarity), (97.0 %) and (96.8 %). Levels of DNA–DNA relatedness between strain YIM kkny3 and the type strains of , and were 12.4, 10.6 and 15.7 %, respectively. The combination of phylogenetic analysis, genotypic data, phenotypic characteristics and chemotaxonomic differences indicated that strain YIM kkny3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM kkny3 (=CCTCC AA 207023=DSM 19797=KCTC 13193).

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2009-08-01
2024-04-18
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Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China
Int J Syst Evol Microbiol 59, 2058 (2009); https://doi.org/10.1099/ijs.0.003624-0
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