RT Journal Article SR Electronic(1) A1 Doerfert, Sebastian N. A1 Reichlen, Matthew A1 Iyer, Prabha A1 Wang, Mingyu A1 Ferry, James G.YR 2009 T1 Methanolobus zinderi sp. nov., a methylotrophic methanogen isolated from a deep subsurface coal seam JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 5 SP 1064 OP 1069 DO https://doi.org/10.1099/ijs.0.003772-0 PB Microbiology Society, SN 1466-5034, AB A methanogenic organism from the domain Archaea (SD1T) was isolated from saline water released from a coal seam located 926 m below the surface via a methane-producing well near Monroe, Louisiana, USA. Growth and methanogenesis were supported with methanol, monomethylamine, dimethylamine or trimethylamine, but not with dimethylsulfide, formate, acetate or H2/CO2. Cells grew in high-salt minimal medium but growth was stimulated with yeast extract or tryptone. Cells were single, non-motile, irregular coccoids 0.5–1.0 μm in diameter and the cell wall contained protein. Conditions for the maximum rate of growth were 40–50 °C, 0.2–0.6 M NaCl, 100–≥200 mM MgCl2, and pH 7.0–8.0. The G+C content of the genomic DNA was 42±1mol %. A comparison of 16S rRNA gene sequences indicated that strain SD1T was most closely related to Methanolobus oregonensis DSM 5435T with 96 % gene sequence similarity. It is proposed that strain SD1T represents a novel species, Methanolobus zinderi sp. nov. The type strain is SD1T (=ATCC BAA-1601T=DSM 21339T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.003772-0