1887

Abstract

Two novel spore-forming lactic acid bacteria, strains SL213 and SL1213, were isolated from vineyard soils in Korea. Cells of both isolates were rod-shaped bacilli and contained subterminal, ellipsoidal spores. Strains were facultatively anaerobic, catalase-positive, oxidase-negative and motile with single flagella. Diaminopimelic acid, glucose and galactose were detected in whole-cell hydrolysates. Major fatty acids found in the strains were anteiso-C, iso-C, iso-C, C and anteiso-C. The G+C contents of the DNA were 46.1 and 46.3 mol%. 16S rRNA gene sequences from the two strains were almost identical (99.9 %) and placed them in the genus , according to phylogenetic analysis. The type strains most closely related to SL213 were ATCC 7050 and ATCC 14574, with 16S rRNA gene sequence similarities of 96.9 and 95.9 %, respectively. Levels of DNA–DNA relatedness between strain SL213 and strain SL1213, ATCC 7050 and ATCC 14574 were 92.5, 49.0 and 27.5 %, respectively. On the basis of 16S rRNA gene sequences and chemotaxonomic and phenotypic evidence given in this study, we report that SL213 represents a novel species, for which the name sp. nov. is proposed. The type strain is SL213 (=KCTC 13078 =JCM 14638).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003913-0
2009-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2226.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003913-0&mimeType=html&fmt=ahah

References

  1. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M.(2005).Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef] [Google Scholar]
  2. Alkan, H., Baysal, Z., Uyar, F. & Dogru, M.(2007). Production of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastes. Appl Biochem Biotechnol 136, 183–192.[CrossRef] [Google Scholar]
  3. Bae, S. S., Lee, J. H. & Kim, S. J.(2005).Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55, 1211–1215.[CrossRef] [Google Scholar]
  4. Becker, M. E. & Pederson, C. S.(1950). The physiological characters of Bacillus coagulans (Bacillus thermoacidurans). J Bacteriol 59, 717–725. [Google Scholar]
  5. Chang, Y. H., Kim, J. K., Kim, H. J., Kim, W. Y., Kim, Y. B. & Park, Y. H.(2001). Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies. Antonie van Leeuwenhoek 80, 193–199.[CrossRef] [Google Scholar]
  6. Chang, Y. H., Han, J., Chun, J. S., Lee, K. C., Rhee, M. S., Kim, Y. B. & Bae, K. S.(2002).Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int J Syst Evol Microbiol 52, 377–381. [Google Scholar]
  7. Cohn, F.(1872). Untersuchungen ϋber Bakterien. Beitr Biol Pflanz 1, 127–244 (in German). [Google Scholar]
  8. De Clerck, E., Rodriguez-Diaz, M., Forsyth, G., Lebbe, L., Logan, N. A. & De Vos, P.(2004). Polyphasic characterization of Bacillus coagulans strains, illustrating heterogeneity within this species, and emended description of the species. Syst Appl Microbiol 27, 50–60.[CrossRef] [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  13. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef] [Google Scholar]
  14. Gatson, J. W., Benz, B. F., Chandrasekaran, C., Satomi, M., Venkateswaran, K. & Hart, M. E.(2006).Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis. Int J Syst Evol Microbiol 56, 1475–1484.[CrossRef] [Google Scholar]
  15. Gibson, T. & Gordon, R. E.(1974). Genus I. Bacillus Cohn 1872, 174. In Bergey's Manual of Determinative Bacteriology, 8th edn, pp. 529–550. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
  16. Gugliandolo, C., Maugeri, T. L., Caccamo, D. & Stackebrandt, E.(2003).Bacillus aeolius sp. nov. a novel thermophilic, halophilic marine Bacillus species from Eolian Islands (Italy). Syst Appl Microbiol 26, 172–176.[CrossRef] [Google Scholar]
  17. Hammer, B. W.(1915). Bacteriological studies on the coagulation of evaporated milk. Iowa Agric Exp Stn Res Bull 19, 119–131. [Google Scholar]
  18. Heyrman, J., Rodríguez-Díaz, M., Devos, J., Felske, A., Logan, N. A. & De Vos, P.(2005).Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 55, 111–117.[CrossRef] [Google Scholar]
  19. Horowitz-Wlassowa, L. M. & Nowotelnow, N. W.(1932). Über eine sporogenes Milchsaurebakterienart, Lactobacillus sporogenes n. sp. Zentralbl Bakteriol II Abt 87, 331 (in German). [Google Scholar]
  20. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  21. Kotay, S. M. & Das, D.(2007). Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge. Bioresour Technol 98, 1183–1190.[CrossRef] [Google Scholar]
  22. Logan, N. A., Lebbe, L., Verhelst, A., Goris, J., Forsyth, G., Rodriguez-Diaz, M., Heyndrickx, M. & De Vos, P.(2002).Bacillus luciferensis sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 52, 1985–1989.[CrossRef] [Google Scholar]
  23. Palmisano, M. M., Nakamura, L. K., Duncan, K. E., Istock, C. A. & Cohan, F. M.(2001).Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona. Int J Syst Evol Microbiol 51, 1671–1679.[CrossRef] [Google Scholar]
  24. Patel, M. A., Ou, M. S., Harbrucker, R., Aldrich, H. C., Buszko, M. L., Ingram, L. O. & Shanmugam, K. T.(2006). Isolation and characterization of acid-tolerant, thermophilic bacteria for effective fermentation of biomass-derived sugars to lactic acid. Appl Environ Microbiol 72, 3228–3235.[CrossRef] [Google Scholar]
  25. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef] [Google Scholar]
  26. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  27. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  28. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K.(1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus. Int J Syst Bacteriol 47, 289–298.[CrossRef] [Google Scholar]
  29. Shivaji, S., Chaturvedi, P., Suresh, K., Reddy, G. S., Dutt, C. B., Wainwright, M., Narlikar, J. V. & Bhargava, P. M.(2006).Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol 56, 1465–1473.[CrossRef] [Google Scholar]
  30. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  31. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  32. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  34. Yanagida, F., Suzuki, K., Kozaki, M. & Komagata, K.(1997). Proposal of Sporolactobacillus nakayamae subsp. nakayamae sp. nov., subsp. nov., Sporolactobacillus nakayamae subsp. racemicus subsp. nov., Sporolactobacillus terrae sp. nov., Sporolactobacillus kofuensis sp. nov., and Sporolactobacillus lactosus sp. nov. Int J Syst Bacteriol 47, 499–504.[CrossRef] [Google Scholar]
  35. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H.(2003).Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53, 1297–1303.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003913-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003913-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error