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Abstract

A moderately halophilic, Gram-positive-staining, endospore-forming, rod-shaped and strictly aerobic bacterium, designated strain NHBX5, was isolated from Lake Nanhuobuxun in China. Strain NHBX5 was able to grow at NaCl concentrations of 0–22 % (w/v) (optimally at 7 %, w/v), at pH 6.0–9.0 (optimally at pH 7.5) and at temperatures of 10–45 °C (optimally at 37 °C). The cell-wall peptidoglycan of strain NHBX5 contained -diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C and anteiso-C. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and a glycolipid. The genomic DNA G+C content of strain NHBX5 was 44.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NHBX5 was most closely related to EN8d (98.6 % gene sequence similarity). The level of DNA–DNA relatedness between strain NHBX5 and CGMCC 1.6199 was 34.6 %. Based on the data presented, strain NHBX5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NHBX5 (=DSM 19340 =CGMCC 1.6502). In addition, an emended description of the genus is presented.

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2009-07-01
2024-03-29
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Scanning electron micrograph of cells of strain NHBX5 . Bar, 2.0 µm.

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Cellular fatty acid profiles of strain NHBX5 and EN8d . [PDF](50 KB)

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