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Volume 59, Issue 6

sp. nov., isolated from a placenta of a sow following an abortion Free

Abstract

A Gram-positive, short diphtheroid-shaped organism was isolated from a sow's placenta of an abortion. This novel isolate, strain Murakami, was examined physiologically, chemotaxonomically and phylogenetically. Cells had an irregular V-shaped or palisade arrangement. Colonies appeared translucent on TMVL agar. Cells were strictly anaerobic, negative for catalase and gelatin decomposition and positive for nitrate reduction and soluble starch hydrolysis. Fourteen sugars including glucose were utilized as carbon sources for growth, but 15 sugars including arabinose were not. -Galactosidase, -galactosidase, -glucosidase and leucine arylamidase were produced, but -glucosidase was not. Fermentation products were lactic, succinic and acetic acids. Sugars of whole cells consisted of rhamnose and ribose. The amino-acid composition of the peptidoglycan was glutamic acid, alanine and lysine in the molar ratio of 1 : 2 : 1. The main fatty acid components of whole cells were C, C, C 7 and C 9. The bacterial menaquinone was MK-10(H). The polar lipids were phosphatidylethanolamine and two unknown phosphatidylinositol mannosides. The G+C content of the genomic DNA of strain Murakami was 63.8 mol%. Phylogenetic analysis of 16S rRNA gene sequences from strain Murakami and other members of the genus supported the phenotypic findings that strain Murakami represents a novel species, for which the name sp. nov. is proposed. The type strain is Murakami (=ATCC BAA-1522 =DSM 19515 =JCM 14813).

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2009-06-01
2024-04-23
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References

  1. Azuma, R. & Ito, T.(1987). Botulism in waterfowl and distribution of Clostridium botulism Type C in Japan. In Avian Botulism: an International Perspective, pp. 167–187. Edited by M. L. Eklund & V. R. Dowell. Springfield, IL: C. C. Thomas.
  2. Azuma, R. & Suto, T.(1970). Validity of transfer of the taxonomical position of Corynebacterium pseudopyogenes from genus Corynebacterium to genus Actinomyces. In Culture Collections of Microorganisms (Proceedings of the 1st International Conference on Culture Collections), pp. 493–505. Edited by H. Iizuka & T. Hasegawa. Tokyo: University of Tokyo Press.
  3. Azuma, R., Yamanaka, H., Miyagawa, E., Suto, T. & Ito, Y.(1979). Isolation of Selenomonas spp. from lesions and non-digestive organs of cows, pigs and man. Natl Inst Anim Health Q (Tokyo) 19, 32–39. [Google Scholar]
  4. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  5. Collins, M. D. & Jones, D.(1981). Distribution of isoprenoid quinine structural types in bacteria and their taxonomic implications. Microbiol Rev 45, 316–354. [Google Scholar]
  6. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  7. Cote, R.(1992). Media formulation 1402, MVL. In American Type Culture Collection Catalog of Bacteria and Fungi, 18th edn, p. 472. Edited by R. Ghena, P. Pienta & R. Cote. Rockville, MD: American Type Culture Collection.
  8. Cowan, S. T.(1974).Cowan & Steel's Manual for the Identification of Medical Bacteria, 2nd edn. London: Cambridge University Press.
  9. Ezaki, T., Saidi, S. M., Liu, S. L., Hashimoto, Y., Yamamoto, H. & Yabuuchi, E.(1990). Rapid procedure to determine the DNA base composition from small amounts of gram positive bacteria. FEMS Microbiol Lett 55, 127–130. [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Higgins, D. G. & Sharp, P. M.(1988).clustal: a package for performing multiple sequence alignment on a microcomputer. Gene 73, 237–244.[CrossRef] [Google Scholar]
  12. Ikemoto, S., Kuraishi, H., Komagata, K., Azuma, R., Suto, T. & Murooka, H.(1978). Cellular fatty acid composition in Pseudomonas species. J Gen Appl Microbiol 24, 199–213.[CrossRef] [Google Scholar]
  13. Kohn, J.(1953). A preliminary report of a new gelatin liquefaction method. J Clin Pathol 6, 249[CrossRef] [Google Scholar]
  14. Kudo, T.(2001). The polar lipids. In Manual of Taxonomy and Identification on Bacteria, pp. 135–144. Edited by K. Suzuki, A. Hiraishi & A. Yokota. Tokyo: Springer (in Japanese).
  15. Lechevalier, M. P. & Lechevalier, H. A.(1980). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  16. Lehnen, A., Busse, H.-J., Frölich, K., Krasinska, M., Kämpfer, P. & Speck, S.(2006).Arcanobacterium bialowienzense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983. Int J Syst Evol Microbiol 56, 861–866.[CrossRef] [Google Scholar]
  17. Saiki, R. K., Gelfand, D. H., Stoffe, S., Scharl, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, A.(1988). Primer-directed enzymatic amplification of DNA with a thermo-stable DNA polymerase. Science 239, 487–491.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Slack, J. M. & Gerencser, M. A.(1975).Actinomyces, Filamentous Bacteria. Biology and Pathogenicity. Minneapolis: Burgess.
  20. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  21. Tabaru, H., Kadota, E., Yamada, H., Sasaki, N. & Takeuchi, A.(1988). Determination of volatile fatty acids and lactic acid in bovine plasma and ruminal fluid by high performance liquid chromatography. Nippon Juigaku Zasshi 50, 1124–1126.[CrossRef] [Google Scholar]
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Arcanobacterium abortisuis sp. nov., isolated from a placenta of a sow following an abortion
Int J Syst Evol Microbiol 59, 1469 (2009); https://doi.org/10.1099/ijs.0.004465-0
/content/journal/ijsem/10.1099/ijs.0.004465-0
/content/journal/ijsem/10.1099/ijs.0.004465-0
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