%0 Journal Article %A Finster, Kai Waldemar %A Herbert, Rodney Andrew %A Kjeldsen, Kasper Urup %A Schumann, Peter %A Lomstein, Bente Aagaard %T Demequina lutea sp. nov., isolated from a high Arctic permafrost soil %D 2009 %J International Journal of Systematic and Evolutionary Microbiology, %V 59 %N 4 %P 649-653 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.004929-0 %K DMK, demethylmenaquinone %I Microbiology Society, %X Two Gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains SV45T and SV47), isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway, have been characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder Micrococcineae of the order Actinomycetales. DNA–DNA hybridization analyses indicate that strains SV45T and SV47 are closely related (60–69 % relatedness) and belong to the same species, although they show slightly different colony pigmentation. The closest phylogenetic neighbour was Demequina aestuarii JC2054T, with 96 % 16S rRNA gene sequence similarity. Optimum growth of SV45T and SV47 occurred aerobically in the absence of NaCl, but both isolates tolerated up to 2 % NaCl (w/v) in the growth medium. Growth under anaerobic conditions was slow and weak. The peptidoglycan of both isolates was of the A4β type with l-ornithine as the diamino acid and serine as a component of the interpeptide bridge with either d-aspartate (SV45T) or d-glutamate (SV47) as the N-terminal amino acid. The major fatty acids present in both isolates were C15 : 0 (3.2–8.6 %), iso-C16 : 0 (5.0–8.9 %), anteiso-C15 : 0 (59.4–61.5 %), anteiso-C17 : 0 (4.1–8.8 %) and anteiso-C15 : 1 (4.4–6.4 %). Isoprenoid quinones were present at exceptionally low levels in both isolates, and only demethylmenaquinone DMK-9(H4) could be identified with any degree of confidence. Phylogenetic analysis and differences in physiological and biochemical characteristics between the strains and Demequina aestuarii JC2054T indicate that these isolates belong to a novel species within the genus Demequina, for which the name Demequina lutea sp. nov. is proposed. The type strain is SV45T (=LMG 24795T =DSM 19970T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004929-0