RT Journal Article SR Electronic(1) A1 Finster, Kai Waldemar A1 Herbert, Rodney Andrew A1 Kjeldsen, Kasper Urup A1 Schumann, Peter A1 Lomstein, Bente AagaardYR 2009 T1 Demequina lutea sp. nov., isolated from a high Arctic permafrost soil JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 4 SP 649 OP 653 DO https://doi.org/10.1099/ijs.0.004929-0 PB Microbiology Society, SN 1466-5034, AB Two Gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains SV45T and SV47), isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway, have been characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder Micrococcineae of the order Actinomycetales. DNA–DNA hybridization analyses indicate that strains SV45T and SV47 are closely related (60–69 % relatedness) and belong to the same species, although they show slightly different colony pigmentation. The closest phylogenetic neighbour was Demequina aestuarii JC2054T, with 96 % 16S rRNA gene sequence similarity. Optimum growth of SV45T and SV47 occurred aerobically in the absence of NaCl, but both isolates tolerated up to 2 % NaCl (w/v) in the growth medium. Growth under anaerobic conditions was slow and weak. The peptidoglycan of both isolates was of the A4β type with l-ornithine as the diamino acid and serine as a component of the interpeptide bridge with either d-aspartate (SV45T) or d-glutamate (SV47) as the N-terminal amino acid. The major fatty acids present in both isolates were C15 : 0 (3.2–8.6 %), iso-C16 : 0 (5.0–8.9 %), anteiso-C15 : 0 (59.4–61.5 %), anteiso-C17 : 0 (4.1–8.8 %) and anteiso-C15 : 1 (4.4–6.4 %). Isoprenoid quinones were present at exceptionally low levels in both isolates, and only demethylmenaquinone DMK-9(H4) could be identified with any degree of confidence. Phylogenetic analysis and differences in physiological and biochemical characteristics between the strains and Demequina aestuarii JC2054T indicate that these isolates belong to a novel species within the genus Demequina, for which the name Demequina lutea sp. nov. is proposed. The type strain is SV45T (=LMG 24795T =DSM 19970T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004929-0