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Volume 59, Issue 8

sp. nov., isolated from soil from a ginseng field, and emended description of the genus Free

Abstract

A Gram-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 191, was isolated from a soil sample from a ginseng field in Pocheon Province, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 191 belongs to the family and is related to LMG 23001 (97.8 % sequence similarity) and KCTC 12396 (96.9 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-8 and the major fatty acids were iso-C 9 (30.6 %), iso-C (21.6 %) and iso-C (13.0 %), supporting the affiliation of strain Gsoil 191 to the genus . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness values between strain Gsoil 191 and its closest phylogenetic neighbours were below 40 %. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 191 from recognized species of the genus . On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 191 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 191 (=KCTC 12564=DSM 17954=CCUG 52462).

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2009-08-01
2024-04-19
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Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella
Int J Syst Evol Microbiol 59, 1947 (2009); https://doi.org/10.1099/ijs.0.004945-0
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Transmission electron micrograph of a cell of strain Gsoil 191 ( sp. nov.). [PDF](249 KB)

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Two-dimensional thin-layer chromatograms of the polar lipids of strain Gsoil 191 ( sp. nov.). [PDF](93 KB)

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