Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera
Crespo-Medina, Melitza and Chatziefthimiou, Aspassia and Cruz-Matos, Ramaydalis and Pérez-Rodríguez, Ileana and Barkay, Tamar and Lutz, Richard A. and Starovoytov, Valentin and Vetriani, Costantino,, 59, 1497-1503 (2009),
doi = https://doi.org/10.1099/ijs.0.005058-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A mesophilic, aerobic, facultatively chemolithoautotrophic bacterium, designated strain EPR70T, was isolated from hydrothermal fluids from diffuse-flow vents on the East Pacific Rise at  ° 50′ N 10 ° 17′ W. Cells were Gram-negative rods, approximately 0.8–1.0 μm long and 0.3–0.5 μm wide. Strain EPR70T grew at 20–40 °C (optimum 30–35 °C), 1–25 % NaCl (optimum 2.5 %) and pH 5.0–7.5 (optimum pH 5.5). The shortest generation time observed for strain EPR70T was 42 min. Growth occurred under aerobic chemolithoautotrophic conditions in the presence of thiosulfate and CO2. Strain EPR70T grew heterotrophically with acetate or n-alkanes as sole carbon and energy sources, and in complex artificial seawater medium. Nitrate was not used as an electron acceptor. The G+C content of the genomic DNA was 64 mol%. Phylogenetic analysis of the 16S rRNA gene indicated that this organism is a member of the class Gammaproteobacteria, with Salinisphaera shabanensis E1L3AT as its closest relative (94 % sequence similarity). On the basis of phylogenetic analyses based on 16S rRNA, rbcL and alkB genes and physiological analysis, it is proposed that the organism represents a novel species within the genus Salinisphaera, for which the name Salinisphaera hydrothermalis sp. nov. is proposed. The type strain is EPR70T (=DSM 21483T =JCM 15514T).,
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