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Volume 59, Issue 6

sp. nov., isolated from the banks of a river Free

Abstract

As part of an antibiotic-screening programme, an actinomycete, designated strain SPR, was isolated from soil collected from the banks of the Umgeni River, KwaZulu-Natal Province, South Africa. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, almond-shaped, non-motile spores. Strain SPR exhibited antibiosis against various Gram-positive and Gram-negative bacteria, including VanA (a vancomycin-resistant strain), A+ and ATCC 25922. The chemotaxonomic characteristics of the strain, with the exception of the phospholipid pattern, corresponded with those of the members of the family Waksman and Henrici 1943. Furthermore, phylogenetic analysis based on 16S rRNA genes showed that the strain was closely related to members of the genus , which supports its classification in the family . Thus strain SPR represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SPR (=DSM 44925=NRRL B-24448).

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2009-06-01
2024-04-24
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References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Anderson, A. S. & Wellington, E. M. H.(2001). The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51, 797–814.[CrossRef] [Google Scholar]
  3. Atlas, R. M.(2004).Handbook of Microbiological Media, 3rd edn. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  4. Betina, V.(1973). Bioautography in paper and thin-layer chromatography and its scope in the antibiotic field. J Chromatogr 78, 41–51.[CrossRef] [Google Scholar]
  5. Chou, S., Chedore, P. & Kasatiya, S.(1998). Use of gas chromatographic fatty acid and mycolic acid cleavage product determination to differentiate among Mycobacterium genavense, Mycobacterium fortuitum, Mycobacterium simiae and Mycobacterium tuberculosis. J Clin Microbiol 36, 577–579. [Google Scholar]
  6. Chun, J. & Goodfellow, M.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef] [Google Scholar]
  7. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  8. Chung, Y. R., Sung, K. C., Mo, H. K., Son, D. Y., Nam, J. S., Chun, J. & Bae, K. S.(1999).Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Int J Syst Bacteriol 49, 753–758.[CrossRef] [Google Scholar]
  9. Cook, A. E. & Meyers, P. R.(2003). Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 53, 1907–1915.[CrossRef] [Google Scholar]
  10. Euzéby, J.(2008). List of prokaryotic names with standing in nomenclature. http://www.bacterio.cict.fr/
  11. Goodfellow, M.(1989). Maduromycetes. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2509–2551. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  12. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  13. Groth, I., Vettermann, R., Shuetze, B., Schumann, P. & Saiz-Jimenez, C.(1999). Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo). J Microbiol Methods 36, 115–122.[CrossRef] [Google Scholar]
  14. Groth, I., Schütze, B., Boettcher, T., Pullen, C. B., Rodriguez, C., Leistner, E. & Goodfellow, M.(2003).Kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of Streptomyces kifunensis to the genus Kitasatospora as Kitasatospora kifunensis comb. nov., and emended description of Streptomyces aureofasciens Duggar 1948. Int J Syst Evol Microbiol 53, 2033–2040.[CrossRef] [Google Scholar]
  15. Hacène, H. & Lefebvre, G.(1995). AH17, a new non-polyenic antifungal antibiotic produced by a strain of Spirillospora. Microbios 83, 199–205. [Google Scholar]
  16. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  17. Kim, B., Al-Tai, A. M., Kim, S. B., Somasundaram, P. & Goodfellow, M.(2000).Streptomyces thermocoprophilus sp. nov., a cellulose-free endo-xylanase-producing streptomycete. Int J Syst Evol Microbiol 50, 505–509.[CrossRef] [Google Scholar]
  18. Kim, S. B., Seong, C. N., Jeon, S. J., Bae, K. S. & Goodfellow, M.(2004). Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov. Int J Syst Evol Microbiol 54, 211–214.[CrossRef] [Google Scholar]
  19. Klappenbach, J. A., Dunbar, J. M. & Schmidt, T. M.(2000). rRNA operon copy number reflects ecological strategies of bacteria. Appl Environ Microbiol 66, 1328–1333.[CrossRef] [Google Scholar]
  20. Komagata, K. & Suzuki, K.-I.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  21. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  22. Li, W., Lanoot, B., Zhang, Y., Vancanneyt, M., Swings, J. & Liu, Z.(2002).Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. Int J Syst Evol Microbiol 52, 1629–1633.[CrossRef] [Google Scholar]
  23. Li, W. J., Zhang, Y. G., Zhang, Y. Q., Tang, S. K., Xu, P., Xu, L. H. & Jiang, C. L.(2005).Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol 55, 1329–1333.[CrossRef] [Google Scholar]
  24. Locci, R.(1989).Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  25. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In Methods in Enzymology, vol. 12, pp. 195–206. Edited by L. Grossman & K. Moldave. New York: Academic Press.
  26. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  27. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  28. Ping, X., Takahashi, Y., Seino, A., Iwai, Y. & Omura, S.(2004).Streptomyces scabrisporus sp. nov. Int J Syst Evol Microbiol 54, 577–581.[CrossRef] [Google Scholar]
  29. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  30. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989). Bacterial media, antibiotics and bacterial strains. In Molecular Cloning, a Laboratory Manual, 2nd edn, Appendix A, pages A1 and A4. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  31. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  32. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  33. Shirling, E. B. & Gottlieb, D.(1972). Cooperative description of type strains of Streptomyces. Int J Syst Bacteriol 22, 265–394.[CrossRef] [Google Scholar]
  34. Skarbek, J. D. & Brady, L. R.(1978).Streptomyces cavourensis sp. nov. (nom. rev.) and Streptomyces cavourensis subsp. washingtonensis subsp. nov., a chromomycin-producing subspecies. Int J Syst Bacteriol 28, 45–53.[CrossRef] [Google Scholar]
  35. Takahashi, K. & Nei, M.(2000). Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17, 1251–1258.[CrossRef] [Google Scholar]
  36. Waksman, S. A. & Henrici, A. T.(1943). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341. [Google Scholar]
  37. Wang, Y. & Zhang, Z.(2000). Comparative sequence analyses reveal frequent occurrence of short segments containing an abnormally high number of non-random base variations in bacterial rRNA genes. Microbiology 146, 2845–2854. [Google Scholar]
  38. WHO(2008). Report: Global Tuberculosis Control. http://www.who.int
  39. Xu, P., Li, W.-J., Wu, W.-L., Wang, D., Xu, L.-H. & Jiang, C.-L.(2004).Streptomyces hebeiensis sp. nov. Int J Syst Evol Microbiol 54, 727–731.[CrossRef] [Google Scholar]
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Streptomyces polyantibioticus sp. nov., isolated from the banks of a river
Int J Syst Evol Microbiol 59, 1302 (2009); https://doi.org/10.1099/ijs.0.006171-0
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vol. , part 6, pp. 1302 - 1309

Southern blot showing the rRNA operon copy number for strain SPR . [PDF](20 KB)

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