1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 6

sp. nov., isolated from an evaporating lagoon in the Cuatro Ciénegas valley in Coahuila, Mexico Free

Abstract

Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1N and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus , with R-20805 as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C C 7 and summed feature a (C 7 and/or C 6)] of strains 1N and 3N supported their affiliation with the genus . The DNA–DNA reassociation values with respect to LMG 23201 and other closely related species were <15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1N, from the five phylogenetically closest species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name sp. nov. is proposed. The type strain is 1N (=LMG 24676=CIP 109853).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.006189-0
2009-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1416.html?itemId=/content/journal/ijsem/10.1099/ijs.0.006189-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H.(2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef] [Google Scholar]
  2. Bozal, N., Montes, M. J. & Mercadé, E.(2007).Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an Antarctic environment. Int J Syst Evol Microbiol 57, 2609–2612.[CrossRef] [Google Scholar]
  3. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam-Syed-Mohideen, A. S., McGarrell, D. M., Bandela, A. M., Cardenas, E., Garrity, G. M. & Tiedje, J. M.(2007). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res 35, (Database issue), D169–D172.[CrossRef] [Google Scholar]
  4. Estrada-De Los Santos, P., Bustillos-Cristales, R. & Caballero-Mellado, J.(2001).Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl Environ Microbiol 67, 2790–2798.[CrossRef] [Google Scholar]
  5. Kielwein, G.(1971). Die Isolierung und Differenzierung von Pseudomonaden aus Lebensmitteln. Arch Lebensmittelhyg 22, 29–37 (in German). [Google Scholar]
  6. King, E. O., Ward, M. K. & Raney, D. E.(1954). Two simple media for the demonstration of pycocyanin and fluorescein. J Lab Clin Med 44, 301–307. [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  8. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester, UK: Wiley.
  9. Liu, S. L., Hessel, A. & Sanderson, K. E.(1993). Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria. Proc Natl Acad Sci U S A 90, 6874–6878.[CrossRef] [Google Scholar]
  10. Matushek, M. G., Bonten, M. J. & Hayden, M. K.(1996). Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis. J Clin Microbiol 34, 2598–2600. [Google Scholar]
  11. Migula, W.(1894). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238 (in German). [Google Scholar]
  12. Moore, E. R. B., Tindall, B. J., Martins Dos Santos, V. P., Pieper, D. H., Ramos, J.-L. & Palleroni, N.(2006). Nonmedical Pseudomonas. In The Prokaryotes: a Handbook on the Biology of Bacteria, pp. 643–703. Edited by M. Dworkin and others. New York: Springer.
  13. Romanenko, L. A., Uchino, M., Tebo, B. M., Tanaka, N., Frolova, G. M. & Mikhailov, V. V.(2008).Pseudomonas marincola sp. nov., isolated from marine environments. Int J Syst Evol Microbiol 58, 706–710.[CrossRef] [Google Scholar]
  14. Spiers, A. J., Buckling, A. & Rainey, P.(2000). The causes of Pseudomonas diversity. Microbiology 146, 2345–2350. [Google Scholar]
  15. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kampfer, P., Maiden, M. C. J., Nesme, X., Rossello-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  16. Vandamme, P., Pot, B., Gillis, M., de Vos, P., Kersters, K. & Swings, J.(1996). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438. [Google Scholar]
  17. Vanparys, B., Heylen, K., Lebbe, L. & De Vos, P.(2006).Pseudomonas peli sp. nov. and Pseudomonas borbori sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 56, 1875–1881.[CrossRef] [Google Scholar]
  18. Versalovic, J., Koeuth, T. & Lupski, J. R.(1991). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19, 6823–6831.[CrossRef] [Google Scholar]
  19. Versalovic, J., Schneider, M., de Brujin, F. J. & Lupski, J. R.(1994). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40. [Google Scholar]
  20. Yamamoto, S., Kasai, H., Arnold, D. L., Jackson, R. W., Vivian, A. & Harayama, S.(2000). Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146, 2385–2394. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.006189-0
Loading
Pseudomonas cuatrocienegasensis sp. nov., isolated from an evaporating lagoon in the Cuatro Ciénegas valley in Coahuila, Mexico
Int J Syst Evol Microbiol 59, 1416 (2009); https://doi.org/10.1099/ijs.0.006189-0
/content/journal/ijsem/10.1099/ijs.0.006189-0
/content/journal/ijsem/10.1099/ijs.0.006189-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1416 – 1420

Pulsed-field electrophoresis gel of representative strains of sp. nov. and type and reference strains of known species [ PDF] (104 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error