Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil
Hoshino, Tamotsu and Nakabayashi, Takuto and Hirota, Kikue and Matsuno, Toshihide and Koiwa, Ryuuichi and Fujiu, Seiichi and Saito, Izumi and Tkachenko, Oleg B. and Matsuyama, Hidetoshi and Yumoto, Isao,, 59, 2074-2079 (2009),
doi = https://doi.org/10.1099/ijs.0.006304-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Two Gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, M4-2T and M4-1, were isolated from soil samples collected from Oblast Magadan, Russian Far East, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus Typhula ishikariensis. Strains M4-2T and M4-1 were identified as members of the genus Paenibacillus by phenotypic and phylogenetic analyses based on 16S rRNA gene sequences. The strains contained anteiso-C15 : 0 as the major fatty acid (63.0–64.7 %) and MK-7 as the major isoprenoid quinone. The DNA G+C contents were 42.8 and 41.7 mol%, respectively. 16S rRNA gene sequence analysis showed that strains M4-2T and M4-1 exhibited high similarities with Paenibacillus macquariensis DSM 2T (99.5 and 99.7 %, respectively) and Paenibacillus antarcticus LMG 22078T (99.4 and 99.5 %, respectively). There were no clear differences in the phenotypic characteristics and chemotaxonomic and phylogenetic data between the novel isolates and P. macquariensis DSM 2T. DNA–DNA hybridization experiments between strain M4-2T and P. macquariensis DSM 2T and P. antarcticus LMG 22078T revealed reassociation values of 56 and 49 %, respectively. Multilocus sequence analysis confirmed the differences between the new isolates and reference strains that were observed with the DNA–DNA hybridization studies. On the basis of the results described above, it is proposed that the isolates represent a novel subspecies of P. macquariensis, Paenibacillus macquariensis subsp. defensor subsp. nov. The type strain is M4-2T (=JCM 14954T=NCIMB 14397T).,
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