RT Journal Article SR Electronic(1) A1 Kämpfer, Peter A1 Lodders, Nicole A1 Warfolomeow, Isabel A1 Busse, Hans-JürgenYR 2009 T1 Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 6 SP 1545 OP 1549 DO https://doi.org/10.1099/ijs.0.006841-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive-staining, coccoid-shaped, oxidase-negative, non-spore-forming, non-motile bacterium, strain KSS-17SeT, was isolated from a metalworking fluid. On the basis of its major fatty acid (ai-C15 : 0) and 16S rRNA gene sequence similarity, the strain grouped with Tessaracoccus bendigoensis and Tessaracoccus flavescens, sharing 95.3 and 97.4 % 16S rRNA gene sequence similarity with the respective type strains. Similarities with other established species of the genera Luteococcus, Propioniferax and Granulicoccus were lower than 95.5 %. The quinone system was characterized by the major menaquinone MK-9(H4). In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and an unknown polar lipid were detected as major compounds. Additionally, three unknown glycolipids and minor amounts of phosphatidylethanolamine, two unknown aminolipids and two unknown polar lipids were detected. Phosphatidylinositol was present only in trace amounts. Predominant polyamines were spermine and spermidine. ll-Diaminopimelic acid was identified as the diagnostic diamino acid in the cell wall. The strain showed clear differences in phenotype (including chemotaxonomic features) from both Tessaracoccus species and members of the other above-mentioned genera. DNA–DNA hybridization between KSS-17SeT and T. bendigoensis Ben-106T and T. flavescens SST-39T yielded similarities of 15.1 and 21.0 %, respectively. It is evident that the organism represents a novel species, for which the name Tessaracoccus lubricantis sp. nov. is proposed. The type strain is KSS-17SeT (=DSM 19926T =CCUG 55516T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.006841-0