RT Journal Article SR Electronic(1) A1 Berestovskaya, Julia Ju. A1 Kotsyurbenko, Oleg R. A1 Tourova, Tatyana P. A1 Kolganova, Tatyana V. A1 Doronina, Nina V. A1 Golyshin, Peter N. A1 Vasilyeva, Lina V.YR 2012 T1 Methylorosula polaris gen. nov., sp. nov., an aerobic, facultatively methylotrophic psychrotolerant bacterium from tundra wetland soil JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_3 SP 638 OP 646 DO https://doi.org/10.1099/ijs.0.007005-0 PB Microbiology Society, SN 1466-5034, AB Three strains of Gram-negative, aerobic, motile bacteria with bipolar flagella were isolated from acidic tundra wetland soils near the city of Vorkuta and from the Chukotka and Yugorsky Peninsulas and designated strains V-022T, Ch-022 and Ju-022. The cells were rod-shaped, 0.5–0.6 µm in width and 1.3–4.5 µm in length and reproduced by irregular fission. These bacteria were facultative methylotrophs that used methanol, methylamines and a wide range of other sources of carbon and energy such as sugars and polysaccharides, ethanol and amino acids. The isolates used the Calvin–Benson pathway for the assimilation of one-carbon compounds and were unable to fix nitrogen. The new strains were moderately acidophilic and psychrotolerant, capable of growth over a pH range of 4.0 to 7.8, with optimum growth at pH 5.5–6.0. Growth occurred between 4 and 30 °C (optimum 20–25 °C). The principal phospholipid fatty acid was C18 : 1ω7c. The DNA G+C content of strain V-022T was 65.2 mol%. Analysis of the 16S rRNA gene sequences revealed that all three isolates V-022T, Ch-022 and Yu-022 exhibited almost identical 16S rRNA gene sequences (99.9 % gene sequence similarity) and formed a new lineage within the class Alphaproteobacteria. The name Methylorosula polaris is suggested to accommodate this new genus and novel species with strain V-022T ( = DSM 22001T = VKM V-2485T) as the type strain of the type species., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.007005-0