1887

Abstract

Eight bacterial strains isolated from diseased rainbow trout (=5) and Atlantic salmon (=3) were characterized by phenotypic and molecular taxonomic methods. The isolates were negative for the Gram-reaction, non-motile, rod-shaped and catalase- and oxidase-positive. Colonies on solid media were yellow, smooth, shiny and circular with regular edges. Growth occurred at 4–28 °C (optimum, 15 °C) and with 0–3 % NaCl (optimum, 0.5 %). Analysis of the 16S rRNA gene sequence allocated the micro-organisms to the genus , with PSD1-4 and JS6-6 as their closest relatives (96.9 and 97.1 % sequence similarity, respectively). The levels of DNA–DNA hybridization towards these nearest phylogenetic neighbours were below 17.1 %. The DNA G+C contents of strains VQ-6316s and VQ-4836s were 32.5 and 32.3 mol%, respectively. The predominant menaquinone was MK-6 and the major fatty acids were iso-C, anteiso-C, iso-C 9, iso-C 3-OH and summed feature 3 (comprising C 7 and/or C 7 and/or iso-C 2-OH). The eight isolates were classified as representatives of a novel species, sp. nov., with strain VQ-6316s (=CECT 7357=DSM 21068) as the type strain.

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2009-12-01
2024-03-28
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References

  1. Anacker, R. L. & Ordal, E. J.(1955). Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J Bacteriol 70, 738–741. [Google Scholar]
  2. Avendaño-Herrera, R., Magariños, B., López-Romalde, S., Romalde, J. L. & Toranzo, A. E.(2004). Phenotypic characterization and description of two major O-serotypes in Tenacibaculum maritimum strains isolated from marine fishes. Dis Aquat Organ 58, 1–8.[CrossRef] [Google Scholar]
  3. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  4. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  5. Bernardet, J.-F., Vancanneyt, M., Matte-Tailliez, O., Grisez, L., Tailliez, P., Bizet, C., Nowakowski, M., Kerouault, B. & Swings, J.(2005). Polyphasic study of Chryseobacterium strain isolated from diseased aquatic animals. Syst Appl Microbiol 28, 640–660.[CrossRef] [Google Scholar]
  6. Bernardet, J.-F., Hugo, C. & Bruun, B.(2006). The genera Chryseobacterium and Elizabethkingia. In The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 638–676. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  7. Buck, J. D.(1982). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  8. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  9. de Beer, H., Hugo, C. J., Jooste, P. J., Vancanneyt, M., Coenye, T. & Vandamme, P.(2006).Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa. Int J Syst Evol Microbiol 56, 1317–1322.[CrossRef] [Google Scholar]
  10. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  11. Hugo, C. J., Segers, P., Hoste, B., Vancanneyt, M. & Kersters, K.(2003).Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53, 771–777.[CrossRef] [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Ilardi, P. & Avendaño-Herrera, R.(2008). Isolation of Flavobacterium-like bacteria from diseased salmonids cultured in Chile. Bull Eur Assoc Fish Pathol 28, 176–185. [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M.(2004). MEGA3: integrated software for molecular evolutionary genetic analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. MacFaddin, J. F.(1980).Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore, MD: Williams & Wilkins.
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  17. Mudarris, M., Austin, B., Segers, P., Vancanneyt, M., Hoste, B. & Bernardet, J.-F.(1994).Flavobacterium scophthalmum sp. nov., a pathogen of turbot (Scophthalmus maximus L.). Int J Syst Bacteriol 44, 447–453.[CrossRef] [Google Scholar]
  18. Park, M. S., Jung, S. R., Lee, K. H., Lee, M.-S., Do, J. O., Kim, S. B. & Bae, K. B.(2006).Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56, 433–438.[CrossRef] [Google Scholar]
  19. Reynolds, E. S.(1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17, 208–212.[CrossRef] [Google Scholar]
  20. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Spurr, A. R.(1969). A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26, 31–43.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B.(1994). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef] [Google Scholar]
  24. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]
  25. Weon, H.-Y., Kim, B.-Y., Yoo, S.-H., Kwon, S.-W., Stackebrandt, E. & Go, S.-J.(2008).Chryseobacterium soli sp. nov. and Chryseobacterium jejuense sp. nov., isolated from soil samples from Jeju, Korea. Int J Syst Evol Microbiol 58, 470–473.[CrossRef] [Google Scholar]
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vol. , part 12, pp. 3001 - 3005

Transmission electron micrograph of cells of strain VQ-6316s grown on AOA for 48 h at 15°C.

. API ZYM profiles of species of the genus .

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