1887

Abstract

Three rhizobial strains (CCBAU 01393, CCBAU 01389 and CCBAU 03239) isolated from nodules of grown in saline-alkaline soils in the north of China had identical 16S rRNA genes that showed 99.7 and 99.5 % sequence similarities with those of SO2 and USDA 4128, respectively. Phylogenies of the housekeeping genes , and confirmed their distinct position, differing from recognized species. SDS-PAGE of whole-cell soluble proteins and a series of phenotypic and physiological tests allowed us to differentiate the novel group from all closely related recognized species. The levels of DNA–DNA relatedness between strain CCBAU 01393 and SO2 and USDA 4128 were 34.9 and 20.5 %, respectively. Therefore, we propose that strains CCBAU 01393, CCBAU 01389 and CCBAU 03239 represent a novel species, sp. nov., with strain CCBAU 01393 (=LMG 24763=HAMBI 3051) as the type strain. This strain could form effective nodules on , and .

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2009-12-01
2024-03-28
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References

  1. Amarger, N., Macheret, V. & Laguerre, G.(1997).Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules. Int J Syst Bacteriol 47, 996–1006.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993). In Cowan and Steel's Manual for the Identification of Medical Bacteria, pp. 230–231. Cambridge: Cambridge University Press.
  3. Chen, W. F., Guan, S. H., Zhao, C. T., Yan, X. R., Man, C. X., Wang, E. T. & Chen, W. X.(2008). Different Mesorhizobium species associated with Caragana carry similar symbiotic genes and have common host ranges. FEMS Microbiol Lett 283, 203–209.[CrossRef] [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Diouf, A., de Lajudie, P., Neyra, M., Kersters, K., Gillis, M., Martinez-Romero, E. & Gueye, M.(2000). Polyphasic characterization of rhizobia that nodulate Phaseolus vulgaris in West Africa (Senegal and Gambia). Int J Syst Evol Microbiol 50, 159–170.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Frank, B.(1889). Über die Pilzsymbiose der Leguminosen. Ber Dtsch Bot Ges 7, 332–346 (in German). [Google Scholar]
  8. Gao, J. L., Sun, J. G., Li, Y., Wang, E. T. & Chen, W. X.(1994). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan province, China. Int J Syst Bacteriol 44, 151–158.[CrossRef] [Google Scholar]
  9. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lloyd-Macgilp, S. A. & Young, J. P. W.(2001). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef] [Google Scholar]
  10. Graham, P. H., Sadowsky, M. J., Keyser, H. H., Barnet, Y. M., Bradley, R. S., Cooper, J. E., De Ley, D. J., Jarvis, B. D. W., Roslycky, E. B. & other authors(1991). Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int J Syst Bacteriol 41, 582–587.[CrossRef] [Google Scholar]
  11. Gu, C. T., Wang, E. T., Tian, C. F., Han, T. X., Chen, W. F., Sui, X. H. & Chen, W. X.(2008).Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules. Int J Syst Evol Microbiol 58, 1364–1368.[CrossRef] [Google Scholar]
  12. Han, T. X., Wang, E. T., Wu, L. J., Chen, W. F., Gu, J. G., Gu, C. T., Tian, C. F. & Chen, W. X.(2008).Rhizobium multihospitium sp. nov., isolated from multiple legume species native of Xinjiang, China. Int J Syst Evol Microbiol 58, 1693–1699.[CrossRef] [Google Scholar]
  13. Jordan, D. C.(1984). Family III. Rhizobiaceae Conn 1938, 321AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 234–256. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  14. Laguerre, G., Nour, S. M., Macheret, V., Sanjuan, J., Drouin, P. & Amarger, N.(2001). Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147, 981–993. [Google Scholar]
  15. Lindström, K.(1989).Rhizobium galegae, a new species of legume root nodule bacteria. Int J Syst Bacteriol 39, 365–367.[CrossRef] [Google Scholar]
  16. Lu, Y. L., Chen, W. F., Wang, E. T., Guan, S. H., Yan, X. R. & Chen, W. X.(2009). Genetic diversity and biogeography of rhizobia associated with Caragana species in three ecological regions of China. Syst Appl Microbiol 32, 351–361.[CrossRef] [Google Scholar]
  17. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  18. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  19. Munro, H. N.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, 21–132. Edited by H. N. Munro. New York: Academic Press.
  20. Polhill, R. M.(1994). Classification of the Leguminosae and synopsis of legume genera. In Phytochemical Dictionary of the Leguminosae, vol. 1, pp. xxxv–lvii. Edited by F. A. Bisby, J. Buckingham & J. B. Harborne. London: Chapman and Hill.
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  24. Tan, Z. Y., Xu, X. D., Wang, E. T., Gao, J. L., Martinez-Romero, E. & Chen, W. X.(1997). Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol 47, 874–879.[CrossRef] [Google Scholar]
  25. Turner, S. L. & Young, J. P. W.(2000). The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol 17, 309–319.[CrossRef] [Google Scholar]
  26. Ueda, T., Suga, Y., Yahiro, N. & Matsuguchi, T.(1995). Phylogeny of Sym plasmids of rhizobia by PCR-based sequencing of a nodC segment. J Bacteriol 177, 468–472. [Google Scholar]
  27. Vauterin, L. & Vauterin, P.(1992). Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur Microbiol 1, 37–41. [Google Scholar]
  28. Vincent, J. M.(1970). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell Scientific.
  29. Vinuesa, P., Silva, C., Werner, D. & Martínez-Romero, E.(2005). Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineation. Mol Phylogenet Evol 34, 29–54.[CrossRef] [Google Scholar]
  30. Wang, E. T., van Berkum, P., Beyene, D., Sui, X. H., Dorado, O., Chen, W. X. & Martínez-Romero, E.(1998).Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae. Int J Syst Bacteriol 48, 687–699.[CrossRef] [Google Scholar]
  31. Wang, F. Q., Wang, E. T., Liu, J., Chen, Q., Sui, X. H., Chen, W. F. & Chen, W. X.(2007).Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China. Int J Syst Evol Microbiol 57, 1192–1199.[CrossRef] [Google Scholar]
  32. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  33. Zhao, C. T., Wang, E. T., Chen, W. F. & Chen, W. X.(2008). Diverse genomic species and evidences of symbiotic gene lateral transfer detected among the rhizobia associated with Astragalus species grown in the temperate regions of China. FEMS Microbiol Lett 286, 263–273.[CrossRef] [Google Scholar]
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Phenogram derived from UPGMA/SSM analysis and SDS-PAGE analysis of whole-cell soluble proteins of strains CCBAU 01393 , CCBAU 01389 and CCBAU 03239. [PDF](95 KB)

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Phylogenetic trees based on and genes. [PDF](20 KB)

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