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Volume 59, Issue 12

sp. nov., isolated from root nodules of species Free

Abstract

Five strains of bacteria isolated from nodules of and in Yunnan Province of China were classified within the genus in the class . The highest degree of 16S rRNA gene sequence similarity was determined to be to LMG 6125 (99.7 %) and UPM-Ca7 (99.7 %). Polyphasic taxonomic methods including SDS-PAGE of whole-cell soluble proteins, comparative housekeeping sequence analysis of , and , fatty acid profiles and a series of phenotypic and physiological tests allowed us to cluster the five strains into a coherent group while differentiating them from all previously established species. The DNA–DNA relatedness between the representative strain CCBAU 65327 and the type strains of and was 26.5 and 23.4 %, respectively, clearly indicating that strain CCBAU 65327 represents a novel species for which we propose the name sp. nov. Strain CCBAU 65327 (=LMG 24762 =HAMBI 3050) is designated as the type strain, and could nodulate , , , , and in cross-nodulation tests.

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Keyword(s): MP, maximum-parsimony and NJ, neighbour-joining
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2009-12-01
2024-04-19
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Mesorhizobium shangrilense sp. nov., isolated from root nodules of Caragana species
Int J Syst Evol Microbiol 59, 3012 (2009); https://doi.org/10.1099/ijs.0.007393-0
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vol. , part 12, pp. 3012 - 3018

[ PDF] (195KB), containing:

Dendrogram based upon SDS-PAGE of whole-cell soluble proteins.

Comparison of partial (a) and (b) gene phylogenies, showing that the novel group has close relationships with the type strains of , , and and is distantly related to the other species of the genus .

Sequence similarities of 16S rRNA gene, , , , and and DNA relatedness among novel strain CCBAU 65327 and related strains.

Cellular fatty acid compositions of strain CCBAU 65327 and previously reported species of the genus .

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