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Volume 59, Issue 9

gen. nov., sp. nov., a new member of the family isolated from seawater Free

Abstract

An aerobic, pale-orange-pigmented, Gram-stain-negative bacterium, designated strain EM39, was isolated from seawater from the eastern coast of Jeju Island, Korea, and its taxonomic status was established using a polyphasic approach. Comparative 16S rRNA gene sequence studies revealed that strain EM39 formed a distinct lineage within the family and could be distinguished from strains of members of the related genera , and by 16S rRNA gene sequence analysis (similarity values between strain EM39 and related strains were all less than 93.8 %). Cells of strain EM39 were non-gliding, catalase- and oxidase-positive rods that were devoid of flexirubin pigments. Growth was observed at 15–35 °C (optimum, 25–30 °C) and pH 6.5–9.0 (optimum, pH 7.0–8.5). The genomic DNA G+C content was 34.6 mol% and the major respiratory quinone was MK-6. The predominant cellular fatty acids were iso-C, iso-C G and iso-C 3-OH. On the basis of phenotypic and genotypic data, strain EM39 represents a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is EM39 (=KCTC 22298=DSM 21165).

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Keyword(s): ASW, artificial seawater
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2009-09-01
2024-04-25
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References

  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  2. Bowman, J. P. & Nichols, D. S.(2005). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int J Syst Evol Microbiol 55, 1471–1486.[CrossRef] [Google Scholar]
  3. Einen, J. & Øvreås, L.(2006).Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 56, 2455–2461.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  6. Jeon, C. O., Park, W., Ghiorse, W. C. & Madsen, E. L.(2004).Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54, 93–97.[CrossRef] [Google Scholar]
  7. Jung, S.-Y., Kang, S.-J., Lee, M.-H., Lee, S.-Y., Oh, T.-K. & Yoon, J.-H.(2005).Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 55, 1845–1849.[CrossRef] [Google Scholar]
  8. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  9. Kwon, K. K., Lee, H.-S., Jung, H.-B., Kang, J.-H. & Kim, S.-J.(2006a).Yeosuana aromativorans gen. nov., sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae, isolated from estuarine sediment of the South Sea, Korea. Int J Syst Evol Microbiol 56, 727–732.[CrossRef] [Google Scholar]
  10. Kwon, K. K., Lee, S. J., Park, J. H., Ahn, T.-Y. & Lee, H. K.(2006b).Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm. Int J Syst Evol Microbiol 56, 1055–1058.[CrossRef] [Google Scholar]
  11. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  13. Lee, S. D.(2007).Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57, 764–769.[CrossRef] [Google Scholar]
  14. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Kwak, J., Mikhailov, V. V. & Bae, K. S.(2006).Mariniflexile gromovii gen. nov., sp. nov., a gliding bacterium isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 56, 1635–1638.[CrossRef] [Google Scholar]
  16. Nedashkovskaya, O. I., Vancanneyt, M., Kim, S. B., Hoste, B. & Bae, K. S.(2007).Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter. Int J Syst Evol Microbiol 57, 2147–2150.[CrossRef] [Google Scholar]
  17. Pearson, W. R. & Lipman, D. J.(1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef] [Google Scholar]
  18. Reichenbach, H.(1989). Order I. Cytophagales Leadbetter 1974, 99AL. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. C. Holt. Baltimore: Williams & Wilkins.
  19. Reva, O. N., Vyunitskaya, V. A., Reznik, S. R., Kozachko, I. A. & Smirnov, V. V.(1995). Antibiotic susceptibility as a taxonomic characteristic of the genus Bacillus. Int J Syst Bacteriol 45, 409–411.[CrossRef] [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  23. Yang, S.-J. & Cho, J.-C.(2008).Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. Int J Syst Evol Microbiol 58, 315–318.[CrossRef] [Google Scholar]
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Jejuia pallidilutea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater
Int J Syst Evol Microbiol 59, 2148 (2009); https://doi.org/10.1099/ijs.0.007625-0
/content/journal/ijsem/10.1099/ijs.0.007625-0
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