@article{mbs:/content/journal/ijsem/10.1099/ijs.0.008045-0, author = "DiPippo, Jonathan L. and Nesbø, Camilla L. and Dahle, Håkon and Doolittle, W. Ford and Birkland, Nils-Kåre and Noll, Kenneth M.", title = "Kosmotoga olearia gen. nov., sp. nov., a thermophilic, anaerobic heterotroph isolated from an oil production fluid", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "12", pages = "2991-3000", doi = "https://doi.org/10.1099/ijs.0.008045-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.008045-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "NJ, neighbour-joining", keywords = "ME, minimum-evolution", keywords = "ML, maximum-likelihood", abstract = "A novel thermophilic, heterotrophic bacterium, strain TBF 19.5.1T, was isolated from oil production fluid at the Troll B oil platform in the North Sea. Cells of strain TBF 19.5.1T were non-motile rods with a sheath-like structure, or toga. The strain was Gram-negative and grew at 20–80 °C (optimum 65 °C), pH 5.5–8.0 (optimum pH 6.8) and NaCl concentrations of 10–60 g l−1 (optimum 25–30 g l−1). For a member of the order Thermotogales, the novel isolate is capable of unprecedented growth at low temperatures, with an optimal doubling time of 175 min (specific growth rate 0.24 h−1) and a final optical density of >1.4 when grown on pyruvate at 37 °C. Various carbohydrates, proteinaceous compounds and pyruvate served as growth substrates. Thiosulfate, but not elemental sulfur, enhanced growth of the isolate. Sulfate also enhanced growth, but sulfide was not produced. The strain grew in the presence of up to approximately 15 % oxygen, but only if cysteine was included in the medium. Growth of the isolate was inhibited by acetate, lactate and propionate, while butanol and malate prevented growth. The major fermentation products formed on maltose were hydrogen, carbon dioxide and acetic acid, with traces of ethanol and propionic acid. The G+C content of the genomic DNA was 42.5 mol%. Phylogenetic analyses of the 16S and 23S rRNA gene sequences as well as 29 protein-coding ORFs placed the strain within the bacterial order Thermotogales. Based on the phylogenetic analyses and the possession of a variety of physiological characteristics not previously found in any species of this order, it is proposed that the strain represents a novel species of a new genus within the family Thermotogaceae, order Thermotogales. The name Kosmotoga olearia gen. nov., sp. nov. is proposed. The type strain of Kosmotoga olearia is TBF 19.5.1T (=DSM 21960T =ATCC BAA-1733T).", }