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Volume 60, Issue 1

sp. nov., isolated from dry riverbed soil Free

Abstract

Two Gram-staining-negative, rod-shaped, non-spore-forming bacterial strains, 1-2 and 1-4 were isolated from dry riverbed soil collected from the Xietongmen area of Tibet, China. On the basis of 16S rRNA gene sequence similarity, the novel strains were shown to belong to the genus , sharing <95 % sequence similarity with all recognized species of the genus . The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C15 : 0, iso-C 3-OH and summed feature 3 (comprising iso-C 7 and/or C 6). The DNA GC contents were 37.2–37.6 mol%. Chemotaxonomic data supported the affiliation of the two new isolates to the genus and the results of physiological and biochemical tests confirmed that the new strains differed significantly from the recognized species of the genus . Therefore, the new isolates represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 1-2 (=CCTCC AB 206110=KCTC 22438).

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2010-01-01
2024-04-23
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References

  1. An D.-S., Kim S.-G., Ten L. N., Cho C.-H. 2009; Pedobacter daechungensis sp. nov., from freshwater lake sediment in South Korea. Int J Syst Evol Microbiol 59:69–72 [CrossRef]
     [Google Scholar]
  2. Baik K.-S., Park Y.-D., Kim M.-S., Park S.-C., Moon E.-Y., Rhee M.-S., Choi J.-H., Seong C.-N. 2007 Pedobacter koreensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57, 2079–2083. [CrossRef]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
     [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  6. Gallego V., García M. T., Ventosa A. 2006; Pedobacter aquatilis sp. nov., isolated from drinking water, and emended description of the genus Pedobacter . Int J Syst Evol Microbiol 56:1853–1858 [CrossRef]
     [Google Scholar]
  7. Hwang C. Y., Choi D. H., Cho B. C. 2006; Pedobacter roseus sp. nov., isolated from a hypertrophic pond, and emended description of the genus Pedobacter . Int J Syst Evol Microbiol 56:1831–1836 [CrossRef]
     [Google Scholar]
  8. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  9. Kumar S., Tamura K., Nei M. 2004; MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
     [Google Scholar]
  10. Kwon S.-W., Kim B. Y., Lee K.-H., Jang K.-Y., Seok S.-J., Kwon J.-S., Kim W.-G., Weon H. Y. 2007; Pedobacter suwonensis sp. nov., isolated from the rhizosphere of Chinese cabbage ( Brassica campestris ). Int J Syst Evol Microbiol 57:480–484 [CrossRef]
     [Google Scholar]
  11. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–176 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  12. Lee H.-G., Im W.-T., Oh H.-M., Lee S.-T. 2009; Pedobacter composti sp. nov., isolated from compost in South Korea. Int J Syst Evol Microbiol 59:345–349 [CrossRef]
     [Google Scholar]
  13. Margesin R., Spröer C., Schumann P., Schinner F. 2003; Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. Int J Syst Evol Microbiol 53:1291–1296 [CrossRef]
     [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  15. Muurholm S., Cousin S., Pauker S., Brambilla E., Stackebrandt E. 2007 Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp.nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet. Int J Syst Evol Microbiol 57, 2221–2227 [CrossRef]
  16. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
     [Google Scholar]
  17. Roh S. W., Quan Z.-X., Nam Y.-D., Chang H.-W., Kim K.-H., Kim M.-K., Im W.-T., Jin L., Kim S.-H. other authors 2008; Pedobacter agri sp. nov., from soil. Int J Syst Evol Microbiol 58:1640–1643 [CrossRef]
     [Google Scholar]
  18. Shivaji S., Chaturvedi P., Reddy G. S. N., Suresh K. 2005; Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55:1083–1088 [CrossRef]
     [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington DC: American Society for Microbiology;
  20. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov.Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177 [CrossRef]
     [Google Scholar]
  21. Ten L. N., Liu Q.-M., Im W.-T., Lee M., Yang D.-C., Lee S.-T. 2006; Pedobacter ginsengisoli sp. nov., a novel DNase-producing bacterium isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 56:2565–2570 [CrossRef]
     [Google Scholar]
  22. Vanparys B., Heylen K., Lebbe L., De Vos P. 2005; Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1315–1318 [CrossRef]
     [Google Scholar]
  23. Yoon J.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2006; Pedobacter sandarakinus sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1273–1277 [CrossRef]
     [Google Scholar]
  24. Yoon M.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2007a; Pedobacter panaciterrae sp. nov., isolated from soil in South Korea. Int J Syst Evol Microbiol 57:381–386 [CrossRef]
     [Google Scholar]
  25. Yoon J.-H., Ten L. N., Im W.-T., Lee S.-T. 2007b; Pedobacter insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:1999–2003 [CrossRef]
     [Google Scholar]
  26. Yoon J.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2007c; Pedobacter lentus sp. nov. and Pedobacter terricola sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:2089–2095 [CrossRef]
     [Google Scholar]
  27. Yoon J.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2007d; Pedobacter terrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:2462–2466 [CrossRef]
     [Google Scholar]
  28. Zimmermann J. J., Langer L., Cooney C. L. 1990; Specific plate assay for bacterial heparinase. Appl Environ Microbiol 56:3593–3594
     [Google Scholar]
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Pedobacter glucosidilyticus sp. nov., isolated from dry riverbed soil
Int J Syst Evol Microbiol 60, 229 (2010); https://doi.org/10.1099/ijs.0.008060-0
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