RT Journal Article SR Electronic(1) A1 Jean, Wen Dar A1 Huang, Ssu-Po A1 Liu, Tung Yen A1 Chen, Jwo-Sheng A1 Shieh, Wung YangYR 2009 T1 Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 8 SP 1880 OP 1887 DO https://doi.org/10.1099/ijs.0.008235-0 PB Microbiology Society, SN 1466-5034, AB Two agarolytic strains of Gram-negative, heterotrophic, facultatively anaerobic, marine bacteria, designated AAM1T and AAT1T, were isolated from seawater samples collected in the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The two isolates required NaCl for growth and grew optimally at about 25–30 °C, in 2–4 % NaCl and at pH 8. They grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other sugars. The major isoprenoid quinone was Q-8 (79.8–92.0 %) and the major cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH; 26.4–35.6 %), C18 : 1 ω7c (27.1–31.4 %) and C16 : 0 (14.8–16.3 %) in the two strains. Strains AAM1T and AAT1T had DNA G+C contents of 52.9 and 52.4 mol%, respectively. The two strains had a 16S rRNA gene sequence similarity of 98.6 % and shared 84.9–92.4 % sequence similarity with the type strains of Agarivorans albus (91.2–92.4 %), eight Alteromonas species (84.9–87.1 %), two Aestuariibacter species (86.0–87.0 %), Bowmanella denitrificans (86.1–86.7 %), eight Glaciecola species (85.0–87.9 %) and Salinimonas chungwhensis (85.9–86.1 %). Despite their high sequence similarity, strains AAM1T and AAT1T had a DNA–DNA relatedness value of only 4.5 %. The data obtained from these polyphasic taxonomic studies revealed that the two agarolytic isolates could be classified as representatives of two novel species in a new genus, Aliagarivorans gen. nov., with Aliagarivorans marinus sp. nov. [type strain is AAM1T (=BCRC 17888T=JCM 15522T)] as the type species and Aliagarivorans taiwanensis sp. nov. [type strain is AAT1T (=BCRC 17889T=JCM 15537T)] as a second species., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.008235-0