1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 8

gen. nov., sp. nov. and sp. nov., facultatively anaerobic marine bacteria capable of agar degradation Free

Abstract

Two agarolytic strains of Gram-negative, heterotrophic, facultatively anaerobic, marine bacteria, designated AAM1 and AAT1, were isolated from seawater samples collected in the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The two isolates required NaCl for growth and grew optimally at about 25–30 °C, in 2–4 % NaCl and at pH 8. They grew aerobically and could achieve anaerobic growth by fermenting -glucose or other sugars. The major isoprenoid quinone was Q-8 (79.8–92.0 %) and the major cellular fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH; 26.4–35.6 %), C 7 (27.1–31.4 %) and C (14.8–16.3 %) in the two strains. Strains AAM1 and AAT1 had DNA G+C contents of 52.9 and 52.4 mol%, respectively. The two strains had a 16S rRNA gene sequence similarity of 98.6 % and shared 84.9–92.4 % sequence similarity with the type strains of (91.2–92.4 %), eight species (84.9–87.1 %), two species (86.0–87.0 %), (86.1–86.7 %), eight species (85.0–87.9 %) and (85.9–86.1 %). Despite their high sequence similarity, strains AAM1 and AAT1 had a DNA–DNA relatedness value of only 4.5 %. The data obtained from these polyphasic taxonomic studies revealed that the two agarolytic isolates could be classified as representatives of two novel species in a new genus, gen. nov., with sp. nov. [type strain is AAM1 (=BCRC 17888=JCM 15522)] as the type species and sp. nov. [type strain is AAT1 (=BCRC 17889=JCM 15537)] as a second species.

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2009-08-01
2024-04-20
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Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation
Int J Syst Evol Microbiol 59, 1880 (2009); https://doi.org/10.1099/ijs.0.008235-0
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