1887

Abstract

Novel aerobic, Gram-negative bacteria with DNA G+C contents below 50 mol% were isolated from the culturable microbiota associated with the Mediterranean seagrass . 16S rRNA gene sequence analyses revealed that they belong to the genus . Strain IVIA-Po-186 is a strain of the species , showing 99.77 % 16S rRNA gene sequence similarity with the type strain, MMB-1, and sharing all phenotypic characteristics studied. This is the first description of this species forming part of the microbiota of a marine plant. A second strain, designated IVIA-Po-101, was closely related to based on phylogenetic studies. However, it differed in characteristics such as melanin synthesis and tyrosinase, laccase and antimicrobial activities. In addition, strain IVIA-Po-101 was auxotrophic and unable to use acetate. IVIA-Po-101 shared 97.86 % 16S rRNA gene sequence similarity with MMB-1, but the level of DNA–DNA relatedness between the two strains was only 10.3 %. On the basis of these data, strain IVIA-Po-101 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IVIA-Po-101 (=CECT 7378 =NCIMB 14432). A third novel strain, IVIA-Po-185, was phylogenetically distant from all recognized species. It shared the highest 16S rRNA gene sequence similarity (97.4 %) with the type strain of , but the level of DNA–DNA relatedness between the two strains was only 14.5 %. A differential chemotaxonomic marker of this strain in the genus is the presence of the fatty acid C cyclo. Strain IVIA-Po-185 is thus considered to represent a second novel species of the genus, for which the name sp. nov. is proposed. The type strain is IVIA-Po-185 (=CECT 7375 =NCIMB 14435). An emended description of the genus is given based on the description of these two novel species, as well as other species described after the original description of the genus.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008607-0
2010-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/93.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008607-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Baumann L., Baumann P., Allen R. D., Mandel M. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Duarte C. M., Borum J., Short F. T., Walker D. I. 2004; Seagrass ecosystems: their global status and prospects. In Aquatic Ecosystems: Trends and Global Prospects pp 281–294 Edited by Polunin N. V. C. Cambridge: Cambridge University Press;
    [Google Scholar]
  6. EPPO 2004; EPPO Standards – diagnostic protocols for regulated pests. PM7/20 Erwinia amylovora . Bull OEPP/EPPO Bull 34:159–171 [CrossRef]
    [Google Scholar]
  7. Gomez D., Lucas-Elio P., Sanchez-Amat A., Solano F. 2006; A novel type of lysine oxidase: l-lysine-epsilon-oxidase. Biochim Biophys Acta 17641577–1585 [CrossRef]
    [Google Scholar]
  8. Gupta P., Chaturvedi P., Pradhan S., Delille D., Shivaji S. 2006; Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 56:361–364 [CrossRef]
    [Google Scholar]
  9. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V. 2000; Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas -like proteobacteria. Curr Microbiol 41:341–345 [CrossRef]
    [Google Scholar]
  11. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A. 2005; Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55:275–279 [CrossRef]
    [Google Scholar]
  12. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology . pp 655–682 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  13. Kumura K., Minamishima Y., Yamamoto S., Ohashi N., Tamura A. 1991; DNA base composition of Rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. Int J Syst Bacteriol 41:247–248 [CrossRef]
    [Google Scholar]
  14. Lau K. W. K., Ren J. P., Wai N. L. M., Lau S. C. L., Oian P. Y., Wong P. K., Wu M. 2006; Marinomonas ostreistagni sp. nov., isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China. Int J Syst Evol Microbiol 56:2271–2275 [CrossRef]
    [Google Scholar]
  15. Lucas-Elio P., Gomez D., Solano F., Sanchez-Amat A. 2006; The antimicrobial activity of marinocine, synthesized by Marinomonas mediterranea , is due to hydrogen peroxide generated by its lysine oxidase activity. J Bacteriol 188:2493–2501 [CrossRef]
    [Google Scholar]
  16. Macián M. C., Arahal D. R., Garay E., Pujalte M. J. 2005; Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 28:145–150 [CrossRef]
    [Google Scholar]
  17. Marbà N., Duarte C. M., Díaz-Almela E., Terrados J., Álvarez E., Martínez R., Santiago R., Gacia E., Grau A. M. 2005; Direct evidence of imbalanced seagrass ( Posidonia oceanica ) shoot population dynamics in the Spanish Mediterranean. Estuaries 28:53–62 [CrossRef]
    [Google Scholar]
  18. Marco-Noales E., Ordax M., Delgado A., López M. M., Saavedra M. J., Martínez-Murcia A., Garcias N., Marbà N., Duarte C. M. 2006; Microbiota associated with Posidonia oceanica in Western Mediterranean sea.. In Modern Multidisciplinary Applied Microbiology Exploiting Microbes and Their Interactions pp 114–119 Edited by Mendez-Vilas A. New York: Wiley;
    [Google Scholar]
  19. Martinez-Murcia A. J., Anton A. I., Rodriguez-Valera F. 1999; Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli . Int J Syst Bacteriol 49:601–610 [CrossRef]
    [Google Scholar]
  20. Prabagaran S. R., Suresh K., Manorama R., Delille D., Shivaji S. 2005; Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. Int J Syst Evol Microbiol 55:309–313 [CrossRef]
    [Google Scholar]
  21. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [CrossRef]
    [Google Scholar]
  22. Sanchez-Amat A., Solano F. 2005; Genus III. Marinomonas Van Landschoot and De Ley 1984, 91VP (Effective publication Van Landschoot and De Ley 1983, 3071). In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. pp 284–289 New York: Springer;
  23. Sanchez-Amat A., Lucas-Elio P., Fernandez E., Garcia-Borron J. C., Solano F. 2001; Molecular cloning and functional characterization of a unique multipotent polyphenol oxidase from Marinomonas mediterranea . Biochim Biophys Acta 1547104–116 [CrossRef]
    [Google Scholar]
  24. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–1246 [CrossRef]
    [Google Scholar]
  25. Solano F., Garcia E., de Egea E. P., Sanchez-Amat A. 1997; Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl Environ Microbiol 63:3499–3506
    [Google Scholar]
  26. Solano F., Lucas-Elio P., Fernandez E., Sanchez-Amat A. 2000; Marinomonas mediterranea MMB-1 transposon mutagenesis: isolation of a multipotent polyphenol oxidase mutant. J Bacteriol 182:3754–3760 [CrossRef]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  28. Van Landschoot A., De Ley J. 1983; Intra- and intergenic similarities of the rRNA cistrons of Alteromonas , Marinomonas (gen. nov.) and some other Gram negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Wolf P. W., Oliver J. D. 1992; Temperature effects on the viable but non-culturable state of Vibrio vulnificus . FEMS Microbiol Ecol 101:33–39
    [Google Scholar]
  31. Yoon J. H., Kang S. J., Oh T. K. 2005; Marinomonas dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55:2303–2307 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008607-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008607-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error