1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 10

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from a hard water rivulet Free

Abstract

Strains WB 3.3-2, WB 3.2-61, WB 4.1-42 and WB 2.3-68 were isolated from the Westerhöfer Bach hard water rivulet, North Germany. The strains were Gram-staining-negative and catalase-, aminopeptidase- and oxidase-positive. The novel strains lacked flagella and only strain WB3.2-61 showed gliding motility. Isolates WB 3.2-61, WB 4.1-42 and WB 2.3-68 produced flexirubin pigments. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strains showed <98.2 % similarity to the type strains of all recognized species of the genus . Strains WB 3.3-2 and WB 4.1-42 shared 96.3 % sequence similarity and were only distantly related to the type strains of all of the members of the genus . Strain WB 3.2-61 branched adjacent to DSM 15094 (98.0 %), while strain WB 2.3-68 was a neighbour of DSM 3660 (97.1 %). On R2A medium, iso-C was the common major fatty acid; fatty acids C, C, iso-C 3-OH, iso-C 9, iso-C 3-OH and summed feature 3 (comprising C 7 and/or iso-C 2-OH) occurred in all strains though sometimes in low amounts. Metabolic properties revealed clear differences between the four isolates as well as between the isolates and their nearest phylogenetic neighbours. The lack of close relatedness was confirmed by Riboprinter and MALDI-TOF analyses of cell extracts. On the basis of a high number of phenotypic differentiating properties and phylogenetic uniqueness, four novel species are proposed with the following names: (type strain WB 3.3-2=DSM 21788=CIP 109865), (type strain WB 4.1-42=DSM 21790=CIP 109867), (type strain WB 2.3-68=DSM 21789=CIP 109868) and (type strain WB 3.2-61=DSM 21791=CIP 109866).

  • Published Online:
Keyword(s): MALDI-TOF, matrix assisted laser desorption ionisation time-of-flight , ML, maximum-likelihood and NJ, Neighbour-joining
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008771-0
2009-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2610.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008771-0&mimeType=html&fmt=ahah

References

  1. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  3. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  4. Brambilla, E., Päuker, O., Cousin, S., Steiner, U., Reimer, A. & Stackebrandt, E.(2007). High phylogenetic diversity of Flavobacterium spp. isolated from a hardwater creek, Harz Mountains, Germany. Org Divers Evol 7, 145–154.[CrossRef] [Google Scholar]
  5. Cousin, S., Päuker, O. & Stackebrandt, E.(2007).Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int J Syst Evol Microbiol 57, 243–249.[CrossRef] [Google Scholar]
  6. Cousin, S., Brambilla, E., Yang, J. & Stackebrandt, E.(2008). Culturable aerobic bacteria from the upstream region of a karst water rivulet. Int Microbiol 11, 91–100. [Google Scholar]
  7. DSMZ(2001).Catalogue of Strains, 7th edn. Braunschweig, Germany: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH. (http://www.dsmz.de/microorganisms/media_list.php).
  8. Dye, D. W.(1968). A taxonomic study of the genus Erwinia. I. The ‘Amylovora’ group. N Z J Sci 11, 590–607. [Google Scholar]
  9. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Phillips, G. B. (editors)(1981).Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  10. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. Miller, L. T.(1982). Single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  13. Muurholm, S., Cousin, S., Päuker, O., Brambilla, E. & Stackebrandt, E.(2007).Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet. Int J Syst Evol Microbiol 57, 2221–2227.[CrossRef] [Google Scholar]
  14. Reichenbach, H. & Dworkin, M.(1981a). The order Myxobacterales. In The Prokaryotes, pp. 328–355. Edited by M. Starr, H. Stolp, H. Trüper, A. Balows & H. Schlegel. New York: Springer-Verlag.
  15. Reichenbach, H. & Dworkin, M.(1981b). The order Cytophagales (with addenda on the genera Herpetosiphon, Saprospira, and Flexithrix). In The Prokaryotes, pp. 356–379. Edited by M. Starr, H. Stolp, H. Trüper, A. Balows & H. Schlegel. New York: Springer-Verlag.
  16. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  18. Stackebrandt, E., Lang, E., Cousin, S., Päuker, O., Brambilla, E., Kroppenstedt, R. & Lünsdorf, H.(2007).Deefgea rivuli gen. nov., sp. nov., a member of the class Betaproteobacteria. Int J Syst Evol Microbiol 57, 639–645.[CrossRef] [Google Scholar]
  19. Stackebrandt, E., Frühling, F., Cousin, S., Brambilla, E., Lünsdorf, H. & Verbarg, S.(2008).Methylibium subsaxonicum spec. nov., a betaproteobacterium isolated from a hardwater rivulet. Curr Microbiol 56, 298–305.[CrossRef] [Google Scholar]
  20. Tamaki, H., Hanada, S., Kamagata, Y., Nakamura, K., Nomura, N., Nakano, K. & Matsumura, M.(2003).Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53, 519–526.[CrossRef] [Google Scholar]
  21. Van Trappen, S., Mergaert, J. & Swings, J.(2003).Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef] [Google Scholar]
  22. Verbarg, S., Frühling, A., Cousin, S., Brambilla, E., Gronow, S., Lünsdorf, H. & Stackebrandt, E.(2008).Biostraticola tofi gen. nov., spec. nov., a novel member of the family Enterobacteriaceae. Curr Microbiol 56, 603–608.[CrossRef] [Google Scholar]
  23. Wang, Z.-W., Liu, Y.-H., Dai, X., Wang, B.-J., Jiang, C.-Y. & Liu, S.-J.(2006).Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 439–442.[CrossRef] [Google Scholar]
  24. West, P. A. & Colwell, R. R.(1984). Identification and classification of the Vibrionaceae – an overview. In Vibrios in the Environment, pp. 285–363. Edited by R. R. Colwell. New York: Wiley.
  25. Zhu, F., Wang, S. & Zhou, P.(2003).Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008771-0
Loading
Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet
Int J Syst Evol Microbiol 59, 2610 (2009); https://doi.org/10.1099/ijs.0.008771-0
/content/journal/ijsem/10.1099/ijs.0.008771-0
/content/journal/ijsem/10.1099/ijs.0.008771-0
Loading

Data & Media loading...

Supplements

vol. , part 10, pp. 2610 - 2617

Distance dendrogram of MALDI-TOF analysis of whole cells of type strains showing the position of the four isolates. [ PDF] 328 KB



PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error