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Volume 59, Issue 10

sp. nov., isolated from a solar saltern Free

Abstract

A Gram-negative, aerobic, non-spore-forming, halophilic bacterial strain, NTU-104, was isolated from the Szutsao saltern in southern Taiwan, which was previously used as salt production field. The novel isolate grew optimally at 35–40 °C, at pH 7.5–8.0 and in the presence of 5 % (w/v) NaCl. The major fatty acids were C, C 9, C 9, C 3-OH and C. The predominant quinone was Q-9. Phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids. The DNA G+C content was 56.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed the affiliation of the novel isolate to the genus . DNA–DNA hybridization results between strain NTU-104 and the type strains of the most closely related species, and , were 36.4 % and 33.2 %, respectively. On the basis of phenotypic, phylogenetic and genetic analyses, strain NTU-104 is considered to represent a novel species of the genus . The name sp. nov. is proposed, with strain NTU-104 (=BCRC 17809=CGMCC 1.7011=JCM 15751) as the type strain.

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Keyword(s): EPS, exopolysaccharides and PHA, poly-β-hydroxyalkanoate
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2009-10-01
2024-04-26
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Marinobacter szutsaonensis sp. nov., isolated from a solar saltern
Int J Syst Evol Microbiol 59, 2605 (2009); https://doi.org/10.1099/ijs.0.008896-0
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Cellular fatty acids (%) of strain NTU-104 and related species of the genus . [ PDF] 35 KB

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Scanning electron photomicrograph of a cell of strain NTU-104 grown in basal broth. Bar, 1µm.

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Two-dimensional TLC of the polar lipid of strain NTU-104 . PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol.

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Maximum-likelihood tree showing the phylogenetic topology of strain NTU-104 with other species of the genus and related taxa. [ PDF] 182 KB

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Maximum-parsimony tree showing the phylogenetic topology of strain NTU-104 with other species of the genus and related taxa. [ PDF] 183 KB

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