1887

Abstract

A Gram-negative, spiral-shaped, phototrophic, purple non-sulfur bacterial strain, designated JA145, was isolated from a freshwater habitat. Cells of strain JA145 were motile by means of a monopolar flagellum. Intracellular photosynthetic membranes were of the stacked type. Bacteriochlorophyll and the carotenoid lycopene and its glucosides were present as photosynthetic pigments. There was no vitamin requirement for strain JA145. The predominant cellular fatty acids were C 7/C 6 (22.24 %), C (22.97 %) and C 7 (43.24 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JA145 clustered with species of the genus , in the class . The highest sequence similarities of strain JA145 were found with the type strains of (96.12 %) and (96.19 %). Based on the 16S rRNA gene sequence analysis and the morphological and physiological characteristics, strain JA145 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is JA145 (=JCM 14933=KCTC 5703=NBRC 104961).

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2009-08-01
2024-03-29
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. Anil Kumar, P., Srinivas, T. N. R., Sasikala, Ch. & Ramana, Ch. V.(2008a).Allochromatium renukae sp. nov. Int J Syst Evol Microbiol 58, 404–407.[CrossRef] [Google Scholar]
  3. Anil Kumar, P., Aparna, P., Srinivas, T. N. R., Sasikala, Ch. & Ramana, Ch. V.(2008b).Rhodospirillum sulfurexigens sp. nov., a phototrophic alphaproteobacterium requiring a reduced sulfur source for growth. Int J Syst Evol Microbiol 58, 2917–2920.[CrossRef] [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  5. Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O.(2005). PHYML Online–a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33, W557–W559.[CrossRef] [Google Scholar]
  6. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  7. Imhoff, J. F.(2005a). Genus I. Rhodospirillum Molisch 1907, 24AL emend. Imhoff, Petri and Süling 1998, 796. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, Part C, pp. 1–6. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  8. Imhoff, J. F.(2005b). Genus V. Phaeospirillum Imhoff, Petri and Süling 1998, 796VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, Part C, pp. 32–33. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  9. Imhoff, J. F., Süling, J. & Petri, R.(1998a). Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Phaeospirillum gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov. Int J Syst Bacteriol 48, 793–798.[CrossRef] [Google Scholar]
  10. Imhoff, J. F., Süling, J. & Petri, R.(1998b). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Thiocapsa, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef] [Google Scholar]
  11. Lanave, C., Preparata, G., Saccone, C. & Serio, G.(1984). A new method for calculating evolutionary substitution rates. J Mol Evol 20, 86–93.[CrossRef] [Google Scholar]
  12. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  14. Pfennig, N. & Trüper, H. G.(1974). The phototrophic bacteria. In Bergey's Manual of Systematic Bacteriololgy, 8th edn, pp. 24–75. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
  15. Pfennig, N. & Trüper, H. G.(1989). Family Chromatiaceae. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 1637–1653. Edited by J. T. Staley, M. P. Bryant. N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Sanger, F., Nicklen, S. & Coulson, A. R.(1977). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463–5467.[CrossRef] [Google Scholar]
  17. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch. & Ramana, Ch. V.(2007).Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57, 228–232.[CrossRef] [Google Scholar]
  19. Takaichi, S.(1999). Carotenoids and carotenogenesis in anoxygenic photosynthetic bacteria. In The Photochemistry of Carotenoids, pp. 39–69. Edited by H. A. Frank, A. J. Young, G. Britton & R. J. Cogdell. The Netherlands: Kluwer.
  20. Takaichi, S., Maoka, T., Hanada, S. & Imhoff, J. F.(2001). Dihydroxylycopene diglucoside diesters: a novel class of carotenoids from the phototrophic purple sulfur bacteria Halorhodospira abdelmalekii and Halorhodospira halochloris. Arch Microbiol 175, 161–167.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Higgins, D. G., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
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Phase-contrast micrographs of cells of strain JA145 ( sp. nov.). [PDF](20 KB)

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Whole-cell absorption and acetone spectra of strain JA145 . [PDF](37 KB)

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H NMR spectral data. [PDF](77 KB)

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