1887

Abstract

A bacterium, designated strain DC-196, isolated from kitchen refuse compost was analysed by using a polyphasic approach. Strain DC-196 was characterized as a Gram-negative short rod that was catalase- and oxidase-positive, and able to grow at 10–40 °C, pH 6–9 and in NaCl concentrations as high as 3 %. Chemotaxonomically, C was observed to be the predominant cellular fatty acid and ubiquinone 10 (Q10) was the predominant respiratory quinone. The G+C content of the genomic DNA was determined to be 66 mol%. On the basis of the genotypic, phenotypic and chemotaxonomic characteristics, strain DC-196 was assigned to the genus , although with distinctive features. At the time of writing, 16S rRNA gene sequence similarities of 97.6–96.8 % and the low DNA–DNA hybridization values of 38.2–32.2 % with the type strains of the three recognized species confirmed that strain DC-196 represents a novel species of the genus, for which the name sp. nov. is proposed (type strain DC-196=CCUG 55808=LMG 24714).

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2010-01-01
2024-03-28
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References

  1. An D.-S., Im W.-T., Yang H.-C., Lee S.-T. 2006; Shinella granuli gen. nov., sp. nov. and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp. nov. Int J Syst Evol Microbiol 56443–448 [CrossRef]
    [Google Scholar]
  2. Barreiros L., Nogales B., Manaia C. M., Ferreira A. C. S., Pieper D. H., Reis M. A., Nunes O. C. 2003; A novel pathway for mineralization of the thiocarbamate herbicide molinate by a defined bacterial mixed culture. Environ Microbiol 5:944–953 [CrossRef]
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  4. Ferreira da Silva M., Tiago I., Veríssimo A., Boaventura A. R., Nunes O. C., Manaia C. M. 2006; Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant. FEMS Microbiol Ecol 55:322–329 [CrossRef]
    [Google Scholar]
  5. Ferreira da Silva M., Vaz-Moreira I., Gonzalez-Pajuelo M., Nunes O. C., Manaia C. M. 2007; Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiol Ecol 60:166–176 [CrossRef]
    [Google Scholar]
  6. Gaunt M. W., Turner S. L., Rigottier-Gois L., Lloyd-Macgilps S. A., Young J. P. W. 2001; Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51:2037–2048 [CrossRef]
    [Google Scholar]
  7. Im W.-T., Bae H.-S., Yokota A., Lee S. T. 2004; Herbaspirillum chlorophenolicum sp. nov., a 4-chlorophenol-degrading bacterium. Int J Syst Evol Microbiol 54:851–855 [CrossRef]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Lin D. X., Wang E. T., Tang H., Han T. X., He Y. R., Guan S. H., Chen W. X. 2008; Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea . Int J Syst Evol Microbiol 58:1409–1413 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology . pp 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  12. Poly F., Monrozier L. J., Bally R. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152:95–103 [CrossRef]
    [Google Scholar]
  13. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 611–651 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  14. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
    [Google Scholar]
  15. Urdiain M., López-López A., Gonzalo C., Busse H.-J., Langer S., Kämpfer P., Rosselló-Móra R. 2008; Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov. a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium , Rhodobium orientis and Rhodobium gokarnense . Syst Appl Microbiol 31:339–351 [CrossRef]
    [Google Scholar]
  16. Vaz-Moreira I., Nobre M. F., Nunes O. C., Manaia C. M. 2007; Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 571108–1112 [CrossRef]
    [Google Scholar]
  17. Vaz-Moreira I., Silva M. E., Manaia C. M., Nunes O. C. 2008; Diversity of bacterial isolates from commercial and homemade composts. Microb Ecol 55:714–722 [CrossRef]
    [Google Scholar]
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