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Volume 59, Issue 12

sp. nov., a moderately halophilic bacterium from sediment of a saline lake Free

Abstract

A novel, moderately halophilic, Gram-positive bacterium, designated strain XH-22, was isolated from sediment of a saline lake located near Xilinhot, Inner Mongolia Autonomous Region, China. Cells were rod-shaped, endospore-forming and motile. The isolate was able to grow in the presence of 3–20 % (w/v) total salts (optimum, 10 %, w/v), and at 15–40 °C (optimum, 37 °C) and pH 6.0–10.0 (optimum, pH 7.5). Strain XH-22 had diaminopimelic acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, and anteiso-C, C and iso-C as major fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid and two unidentified phospholipids. The DNA G+C content of strain XH-22 was 38.8 mol%. 16S rRNA gene sequence analysis revealed that the novel strain was affiliated with the genus . Levels of 16S rRNA gene sequence similarity between strain XH-22 and the type strains of recognized species ranged from 97.6 % (with ) to 94.9 % (with ). Levels of DNA–DNA relatedness between strain XH-22 and DSM 14868 and DSM 14866 were 32 and 28 %, respectively. Strain XH-22 could be differentiated from recognized species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and genotypic features. On the basis of these results, strain XH-22 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XH-22 (=CCM 7562=CECT 7439=DSM 21756).

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2009-12-01
2024-04-28
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Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake
Int J Syst Evol Microbiol 59, 3068 (2009); https://doi.org/10.1099/ijs.0.009530-0
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