Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone Chen, Yi-Guang and Wang, Yong-Xia and Zhang, Yu-Qin and Tang, Shu-Kun and Liu, Zhi-Xiong and Xiao, Huai-Dong and Xu, Li-Hua and Cui, Xiao-Long and Li, Wen-Jun,, 59, 2708-2713 (2009), doi = https://doi.org/10.1099/ijs.0.009704-0, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-positive, moderately halophilic, alkalitolerant, filamentous, aerobic actinomycete, designated strain JSM 073097T, was isolated from a sea anemone collected from a tidal flat in the South China Sea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and was most closely related to Nocardiopsis kunsanensis HA-9T, Nocardiopsis xinjiangensis YIM 90004T and Nocardiopsis salina YIM 90010T (99.6, 98.5 and 98.1 % similarity, respectively). Phenotypic characteristics and chemotaxonomic data also indicated that strain JSM 073097T was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing white to yellow–white substrate mycelium and white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores. The strain grew in the presence of 1–15 % (w/v) total salts and at pH 6.0–10.5 and 20–35 °C; optimum growth occurred in the presence of 5–7 % (w/v) total salts and at pH 8.5 and 25 °C. Whole-cell hydrolysates of strain JSM 073097T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H4), MK-10(H6) and MK-10(H8). The major cellular fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C16 : 0 and 10-methyl C18 : 0. Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain JSM 073097T was 70.4 mol%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 073097T represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis litoralis sp. nov. is proposed. The type strain is JSM 073097T (=DSM 45168T=KCTC 19473T)., language=, type=