1887

Abstract

Three facultative methylotrophic bacterial strains, designated CBMB127, CBMB145 and CBMB147, were isolated from the rhizosphere soil of rice and characterized. The strains produced indole-3-acetic acid and siderophores, had 1-aminocyclopropane-1-carboxylate deaminase activity and sulfur oxidation property and also methanol dehydrogenase. Phylogenetic analysis based on the 16S rRNA and methanol dehydrogenase () gene sequences showed that was their close relative. The results of the phenotypic, phylogenetic and genotypic analyses showed that strains CBMB127 and CBMB145, with 99.4 % 16S rRNA gene sequence similarity and 99 % DNA–DNA hybridization, could be distinguished from recognized species of . Therefore strain CBMB127 and CBMB145 are considered to represent a novel species of , for which the name sp. nov. is proposed, with CBMB127 (=KACC 13099=NCCB 100233) as the type strain. Strain CBMB147 represents a novel strain of the species .

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2009-11-01
2024-03-29
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References

  1. Anandham, R., Indira Gandhi, P., Madhaiyan, M., Kim, K. A., Yim, W. J., Saravanan, V. S., Chung, J. B. & Sa, T. M.(2007). Thiosulfate oxidation and mixotrophic growth of Methylobacterium oryzae. Can J Microbiol 53, 869–876.[CrossRef] [Google Scholar]
  2. Baldani, V. L. D. & Döbereiner, J.(1980). Host-plant specificity in the infection of cereals with Azospirillum spp. Soil Biol Biochem 12, 433–439.[CrossRef] [Google Scholar]
  3. Colby, J. & Zatman, L. J.(1973). Trimethylamine metabolism in obligate and facultative methylotrophs. Biochem J 132, 101–112. [Google Scholar]
  4. Doronina, N.V. & Trotsenko, Y. A.(1994).Methylophilusleisingerii sp. nov., a new species of restricted facultatively methylotrophic bacteria. Microbiology (English translation of Mikrobiologiia) 63, 298–302. [Google Scholar]
  5. Dunfield, P. F., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A. & Dedysh, S. N.(2003).Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol. Int J Syst Evol Microbiol 53, 1231–1239.[CrossRef] [Google Scholar]
  6. Gerhardt, P. R., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  7. Govorukhina, N. I. & Trotsenko, Y. A.(1991).Methylovorus, a new genus of restricted facultatively methylotrophic bacteria. Int J Syst Bacteriol 41, 158–162.[CrossRef] [Google Scholar]
  8. Green, P. N. & Bousfield, I. J.(1982). A taxonomic study of some Gram-negative facultatively methylotrophic bacteria. J Gen Microbiol 128, 623–638. [Google Scholar]
  9. Jenkins, O., Byrom, D. & Jones, D.(1987).Methylophilus: a new genus of methanol-utilizing bacteria. Int J Syst Bacteriol 37, 446–448.[CrossRef] [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004). MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Madhaiyan, M., Kim, B.-Y., Poonguzhali, S., Kwon, S.-W., Song, M.-H., Ryu, J.-H., Go, S.-J., Koo, B.-S. & Sa, T.-M.(2007).Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int J Syst Evol Microbiol 57, 326–331.[CrossRef] [Google Scholar]
  12. McDonald, I. R. & Murrell, J. C.(1997). The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. Appl Environ Microbiol 63, 3218–3224. [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  14. Poonguzhali, S., Madhaiyan, M. & Sa, T. M.(2006). Cultivation-dependent characterization of rhizobacterial communities from field grown Chinese cabbage Brassica campestris ssp pekinensis and screening of potential plant growth promoting bacteria. Plant Soil 286, 167–180.[CrossRef] [Google Scholar]
  15. Sasser, M.(1990). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  16. Seldin, L. & Dubnau, D.(1985). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef] [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  18. Urakami, T. & Komagata, K.(1986). Emendation of Methylobacillus Yordy and Weaver 1977, a genus for methanol-utilizing bacteria. Int J Syst Bacteriol 36, 502–511.[CrossRef] [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  20. Whittenbury, R., Davies, S. L. & Wilkinson, J. F.(1970). Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol 61, 205–218.[CrossRef] [Google Scholar]
  21. Yordy, J. R. & Weaver, T. Y.(1977).Methylobacillus, a new genus of obligate methylotrophic bacteria. Int J Syst Bacteriol 27, 247–255.[CrossRef] [Google Scholar]
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vol. , part 11, pp. 2904 - 2908

Neighbour-joining phylogenetic tree based on deduced amino acid sequences of products. [PDF](15 KB)



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