1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 9

sp. nov., a halophilic actinomycete isolated from a salt lake Free

Abstract

A halophilic actinomycete strain, designated YIM 91168, was isolated from a salt lake in Xinjiang province, north-west China. The isolate grew at 20–40 °C, pH 5–8 and 6–22 % (w/v) NaCl; there was no growth in the absence of NaCl. The whole-cell hydrolysate contained -diaminopimelic acid, galactose and arabinose. The major fatty acids were iso-C, iso-C and iso-C. MK-9(H) was the predominant menaquinone and the genomic DNA G+C content was 70.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM 91168 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 91168 had highest sequence similarity (95.4 %) with NCIB 12823, and showed lower 16S rRNA gene sequence similarity (93.0–95.1 %) with the other species of the genus . On the basis of evidence from this polyphasic study, the novel species sp. nov. is proposed. The type strain is YIM 91168 (=DSM 45088 =KCTC 19235).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009860-0
2009-09-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2166.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009860-0&mimeType=html&fmt=ahah

References

  1. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  5. Goodfellow, M., Lacey, J., Athalye, M., Embley, T. M. & Bowen, T.(1989).Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139. [Google Scholar]
  6. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  7. Kelly, K. L.(1964).Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Korn-Wendisch, F., Kempf, A., Grund, E., Kroppenstedt, R. M. & Kutzner, H. J.(1989). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.[CrossRef] [Google Scholar]
  10. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2387.[CrossRef] [Google Scholar]
  11. Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  12. Labeda, D. P.(1987). Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.[CrossRef] [Google Scholar]
  13. Lacey, J. & Goodfellow, M.(1975). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88, 75–85.[CrossRef] [Google Scholar]
  14. Lechevalier, H. A. & Lechevalier, M. P.(1970). A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, pp. 393–405. Edited by H. Prauser. Jena: VEB Gustav Fischer.
  15. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  16. Li, W.-J., Xu, P., Schumann, P., Zhang, Y.-Q., Pukall, R., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  17. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  20. Pimentel-Elardo, S. M., Tiro, L. P., Grozdanov, L. & Hentsche, U.(2008).Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 58, 628–632.[CrossRef] [Google Scholar]
  21. Qin, S., Li, J., Zhao, G. Z., Chen, H. H., Xu, L. H. & Li, W. J.(2008).Saccharopolyspora endophytica sp. nov., an endophytic actinomycete isolated from the root of Maytenus austroyunnanensis. Syst Appl Microbiol 31, 352–357.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  26. Tang, S.-K., Tian, X.-P., Zhi, X.-Y., Cai, M., Wu, J.-Y., Yang, L.-L., Xu, L.-H. & Li, W.-J.(2008).Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae. Int J Syst Evol Microbiol 58, 2075–2080.[CrossRef] [Google Scholar]
  27. Tang, S.-K., Wang, Y., Cai, M., Zhi, X.-Y., Lou, K., Xu, L.-H., Jiang, C.-L. & Li, W.-J.(2009).Saccharopolyspora halophila sp. nov., a novel halophilic actinomycete isolated from a saline lake in China. Int J Syst Evol Microbiol 59, 555–558.[CrossRef] [Google Scholar]
  28. Williams, S. T.(1967). Sensitivity of streptomycetes to antibiotics as a taxonomic character. J Gen Microbiol 46, 151–160.[CrossRef] [Google Scholar]
  29. Williams, S. T., Goodfellow, M. & Alderson, G.(1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2463–2468. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams &Wilkins.
  30. Yassin, A. F.(2009).Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma. Int J Syst Evol Microbiol 59, 1148–1152.[CrossRef] [Google Scholar]
  31. Yuan, L.-J., Zhang, Y.-Q., Guan, Y., Wei, Y.-Z., Li, Q.-P., Yu, L.-Y., Li, W.-J. & Zhang, Y.-Q.(2008).Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58, 1180–1185.[CrossRef] [Google Scholar]
  32. Zhang, J., Wu, D., Zhang, J., Liu, Z. & Song, F.(2008).Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58, 1094–1099.[CrossRef] [Google Scholar]
  33. Zhang, J., Wu, D. & Liu, Z.(2009).Saccharopolyspora jiangxiensis sp. nov., isolated from grass-field soil. Int J Syst Evol Microbiol 59, 1076–1081.[CrossRef] [Google Scholar]
  34. Zhou, Z.-H., Liu, Z.-H., Qian, Y.-D., Kim, S. B. & Goodfellow, M.(1998).Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009860-0
Loading
Saccharopolyspora qijiaojingensis sp. nov., a halophilic actinomycete isolated from a salt lake
Int J Syst Evol Microbiol 59, 2166 (2009); https://doi.org/10.1099/ijs.0.009860-0
/content/journal/ijsem/10.1099/ijs.0.009860-0
/content/journal/ijsem/10.1099/ijs.0.009860-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error