1887

Abstract

A facultatively anaerobic, moderately halophilic, Gram-positive, endospore-forming, motile, catalase- and oxidase-positive, rod-shaped bacterium, strain JSM 072002, was isolated from a sea anemone () collected from the South China Sea. Strain JSM 072002 was able to grow with 0.5–15 % (w/v) NaCl and at pH 6.0–10.0 and 15–50 °C; optimum growth was observed with 2–5 % (w/v) NaCl and at pH 7.5 and 35 °C. -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C and anteiso-C. The predominant respiratory quinone was menaquinone 7 and the genomic DNA G+C content was 41.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 072002 should be assigned to the genus and revealed relatively low 16S rRNA gene sequence similarities (<97 %) with the type strains of the three recognized species ( BH030062, 96.8 %; KCTC 3917, 96.7 %; JSM 076056, 96.0 %). The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 072002 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 072002 (=DSM 21186=KCTC 13237). An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009910-0
2010-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/560.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009910-0&mimeType=html&fmt=ahah

References

  1. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  2. Chen Y.-G., Cui X.-L., Pukall R., Li H.-M., Yang Y.-L., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L. 2007; Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57:2327–2332 [CrossRef]
    [Google Scholar]
  3. Chen Y.-G., Zhang Y.-Q., Shi J.-X., Xiao H.-D., Tang S.-K., Liu Z.-X., Huang K., Cui X.-L., Li W.-J. 2009a; Jeotgalicoccus marinus sp. nov., a marine bacterium isolated from sea urchin. Int J Syst Evol Microbiol 59:1625–1629 [CrossRef]
    [Google Scholar]
  4. Chen Y.-G., Zhang Y.-Q., Xiao H.-D., Liu Z.-X., Yi L.-B., Shi J.-X., Zhi X.-Y., Cui X.-L., Li W.-J. 2009b; Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from sea urchin. Int J Syst Evol Microbiol 59:1635–1639 [CrossRef]
    [Google Scholar]
  5. Chen Y.-G., Wang Y.-X., Zhang Y.-Q., Tang S.-K., Liu Z.-X., Xiao H.-D., Xu L.-H., Cui X.-L., Li W.-J. 2009c; Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59:2708–2713 [CrossRef]
    [Google Scholar]
  6. Chen Y.-G., Zhang Y.-Q., Huang H.-Y., Klenk H.-P., Tang S.-K., Huang K., Chen Q.-H., Cui X.-L., Li W.-J. 2009d; Halomonas zhanjiangensis sp. nov., a halophilic bacterium isolated from sea urchin. Int J Syst Evol Microbiol 59:2888–2893 [CrossRef]
    [Google Scholar]
  7. Chen Y.-G., Zhang Y.-Q., Wang Y.-X., Liu Z.-X., Klenk H.-P., Xiao H.-D., Tang S.-K., Cui X.-L., Li W.-J. 2009e; Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from sea anemone. Int J Syst Evol Microbiol 59:3035–3039 [CrossRef]
    [Google Scholar]
  8. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  9. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. 2001; Streptomonospora salina gen. nov., sp. nov. a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  12. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  13. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  14. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  15. Hopwood D. A., Bibb M. J., Chater K. F., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985; Genetic Manipulation of Streptomyces A Laboratory Manual . Norwich: John Innes Foundation;
    [Google Scholar]
  16. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  17. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32
    [Google Scholar]
  18. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [CrossRef]
    [Google Scholar]
  19. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  20. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J. 2005a; Pontibacillus chungwhensis gen. nov., sp. nov. a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
    [Google Scholar]
  21. Lim J.-M., Jeon C. O., Park D.-J., Kim H.-R., Yoon B.-J., Kim C.-J. 2005b; Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus . Int J Syst Evol Microbiol 55:1027–1031
    [Google Scholar]
  22. Mata J. A., Martínez-Cánovas J., Quesada E., Bèjar V. 2002; A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [CrossRef]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  25. Quesada E., Ventosa A., Rodriguez-Valera F., Megias L., Ramos-Cormenzana A. 1983; Numerical taxonomy of moderately halophilic Gram-negative bacteria from hypersaline soils. J Gen Microbiol 129:2649–2657
    [Google Scholar]
  26. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1981; Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol 7:235–243 [CrossRef]
    [Google Scholar]
  27. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  30. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  31. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  33. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  35. Xiao H.-D., Chen Y.-G., Liu Z.-X., Huang K., Li W.-J., Cui X.-L., Zhang L., Yi L.-B. 2009; Phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the Naozhou Island in Zhanjiang, China. Wei Sheng Wu Xue Bao 49:246–250
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009910-0
Loading
/content/journal/ijsem/10.1099/ijs.0.009910-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error