1887

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Volume 59, Issue 10

gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent gene Free

Abstract

Two strains of Gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria were isolated from beechwood blocks during decay by the white-rot fungus and were designated strains BW863 and BW872. They are capable of methylotrophic growth and assimilate carbon via the ribulose-bisphosphate pathway. In addition to methanol, the novel isolates utilized ethanol, pyruvate and malate. Strains BW863 and BW872 are obligately acidophilic, mesophilic organisms capable of growth at pH 3.1–6.5 (with an optimum at pH 4.5–5.0) and at 4–30 °C. Phospholipid fatty acid profiles of these bacteria contain unusually large amounts (about 90 %) of C 7, thereby resembling the profiles of strains. The predominant quinone is Q-10. The DNA G+C content of the novel isolates is 61.8–62.8 mol%. On the basis of 16S rRNA gene sequence similarity, strains BW863 and BW872 are most closely related to the acidophilic methanotroph B2 (96.5–97 %). Comparative sequence analysis of , the gene encoding the large subunit of methanol dehydrogenase, placed the MxaF sequences of the two novel strains in a cluster that is distinct from all previously described MxaF sequences of cultivated methylotrophs. The identity between the MxaF sequences of the acidophilic isolates and those from known alpha-, beta- and gammaproteobacterial methylotrophs was respectively 69–75, 61–63 and 64–67 %. The data therefore suggest that strains BW863 and BW872 represent a novel genus and species of methylotrophic bacteria, for which the name gen. nov., sp. nov. is proposed. Strain BW863 (=DSM 19998 =NCIMB 14408) is the type strain of .

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Keyword(s): ICM, intracytoplasmic membrane , PLFA, phospholipid fatty acid , PMS, phenazine methosulfate , RuBisCO, ribulose-1,5-bisphosphate carboxylase/oxygenase , RuBP, ribulose bisphosphate and SIP, stable-isotope probing
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2009-10-01
2024-04-19
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Methylovirgula ligni gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent mxaF gene
Int J Syst Evol Microbiol 59, 2538 (2009); https://doi.org/10.1099/ijs.0.010074-0
/content/journal/ijsem/10.1099/ijs.0.010074-0
/content/journal/ijsem/10.1099/ijs.0.010074-0
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Striking difference in cell morphology between B2 (left) and strain BW863 (right). Bars, 5 µm.

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Striking difference in cell morphology between B2 (left) and strain BW863 (right). Bars, 5 µm.

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16S rRNA gene-based neighbour-joining tree showing the phylogenetic positions of strains BW863 and BW872 relative to other members of the family and to environmental 16S rRNA gene sequences retrieved by means of the stable-isotope-probing technique from different acidic soils amended with C-methanol. [PDF](45 KB)

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16S rRNA gene-based neighbour-joining tree showing the phylogenetic positions of strains BW863 and BW872 relative to other members of the family and to environmental 16S rRNA gene sequences retrieved by means of the stable-isotope-probing technique from different acidic soils amended with C-methanol. [PDF](45 KB)

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