@article{mbs:/content/journal/ijsem/10.1099/ijs.0.010132-0, author = "Burns, David G. and Janssen, Peter H. and Itoh, Takashi and Minegishi, Hiroaki and Usami, Ron and Kamekura, Masahiro and Dyall-Smith, Mike L.", title = "Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "5", pages = "1173-1176", doi = "https://doi.org/10.1099/ijs.0.010132-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010132-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18–20 % (w/v) NaCl, pH 6.0–8.5 and 37–40 °C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA–DNA hybridization values between Nmn. pharaonis DSM 2160T and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11T (=JCM 14361T =CECT 7526T =DSM 18674T). An emended description of the genus Natronomonas is also provided.", }