RT Journal Article SR Electronic(1) A1 Burns, David G. A1 Janssen, Peter H. A1 Itoh, Takashi A1 Minegishi, Hiroaki A1 Usami, Ron A1 Kamekura, Masahiro A1 Dyall-Smith, Mike L.YR 2010 T1 Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 5 SP 1173 OP 1176 DO https://doi.org/10.1099/ijs.0.010132-0 PB Microbiology Society, SN 1466-5034, AB Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18–20 % (w/v) NaCl, pH 6.0–8.5 and 37–40 °C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA–DNA hybridization values between Nmn. pharaonis DSM 2160T and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11T (=JCM 14361T =CECT 7526T =DSM 18674T). An emended description of the genus Natronomonas is also provided., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010132-0