1887

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain HS286, was isolated from water of the hypersaline Lake Howz-Soltan in Iran. Cells were strictly aerobic, rod-shaped, motile and able to produce ellipsoidal endospores at a central-subterminal position in swollen sporangia. Isolate HS286 grew in a complex medium supplemented with 1–15 % (w/v) NaCl, with optimum growth at 8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain HS286 was closely related to s G-19.1 (99.4 % gene sequence similarity). The other closest species were MSS-402 (96.9 %) and other species of the genus (with 96.7–93.5 % similarity). Strain HS286 had cell-wall peptidoglycan based on -diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C (43.8 %), iso-C (21.4 %), iso-C (9.4 %), anteiso-C (8.7 %) and iso-C (7.0 %) and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids and a glycolipid. The DNA G+C content was 43.0 mol%. All of these features confirmed the placement of isolate HS286 within the genus . However DNA–DNA hybridization between strain HS286 and the only recognized species of the genus , s G-19.1, was 27.3 %, showing unequivocally that the novel isolate constituted a new genospecies. Strain HS286 could be clearly differentiated from and other phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain HS286 constitutes a novel species, for which the name sp. nov. is proposed. The type strain is HS286 (=CCM 7597=JCM 15722).

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2009-10-01
2024-03-28
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