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Abstract
A Gram-positive, moderately halophilic bacterium, designated strain HS286T, was isolated from water of the hypersaline Lake Howz-Soltan in Iran. Cells were strictly aerobic, rod-shaped, motile and able to produce ellipsoidal endospores at a central-subterminal position in swollen sporangia. Isolate HS286T grew in a complex medium supplemented with 1–15 % (w/v) NaCl, with optimum growth at 8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain HS286T was closely related to Thalassobacillus devorans G-19.1T (99.4 % gene sequence similarity). The other closest species were Halobacillus yeomjeoni MSS-402T (96.9 %) and other species of the genus Halobacillus (with 96.7–93.5 % similarity). Strain HS286T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C15 : 0 (43.8 %), iso-C16 : 0 (21.4 %), iso-C14 : 0 (9.4 %), anteiso-C17 : 0 (8.7 %) and iso-C15 : 0 (7.0 %) and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids and a glycolipid. The DNA G+C content was 43.0 mol%. All of these features confirmed the placement of isolate HS286T within the genus Thalassobacillus. However DNA–DNA hybridization between strain HS286T and the only recognized species of the genus Thalassobacillus, T. devorans G-19.1T, was 27.3 %, showing unequivocally that the novel isolate constituted a new genospecies. Strain HS286T could be clearly differentiated from T. devorans and other phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain HS286T constitutes a novel species, for which the name Thalassobacillus cyri sp. nov. is proposed. The type strain is HS286T (=CCM 7597T=JCM 15722T).
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