1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 2

sp. nov., isolated from stream sediment Free

Abstract

An aerobic, yellow-pigmented, Gram-staining-negative bacterium, designated strain H7, was isolated from sediment of Gazwa stream in Jinju city, South Korea. Cells of strain H7 were non-motile, straight rods that produced flexirubin pigments and showed catalase- and oxidase activities. Strain H7 contained iso-C anteiso-C and summed feature 3 (comprising C 7 and/or iso-C 2-OH) as the main fatty acids and menaquinone-6 (MK-6) as the major isoprenoid quinone. The DNA G+C content of strain H7 was 37.2 mol%. Comparative 16S rRNA gene sequence analysis showed that strain H7 formed a distinct phyletic line within the genus . Based on 16S rRNA gene sequence similarities, the novel strain was most closely related to ST-82 and BD-b365 with 97.2 % and 97.0 % gene sequence similarities, respectively. The DNA–DNA relatedness of the novel strain with these species was only 18 % and 14 %, respectively. On the basis of phenotypic data and molecular properties, strain H7 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is H7 (=KACC 12818=DSM 19978).

  • Published Online:
Keyword(s): ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010850-0
2010-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/2/353.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010850-0&mimeType=html&fmt=ahah

References

  1. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. 1923; Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology pp 97–117 Baltimore: Williams & Wilkins;
     [Google Scholar]
  2. Bernardet J.-F., Bowman J. 2006; The genus Flavobacterium . In The Prokaryotes: a Handbook on the Biology of Bacteria . , 3rd edn. vol 7 pp 481–531 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
     [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
     [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  6. Cousin S., Pauker O., Stackebrandt E. 2007; Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int J Syst Evol Microbiol 57:243–249 [CrossRef]
     [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  8. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
     [Google Scholar]
  9. Humphry D. R., George A., Black G. W., Cummings S. P. 2001; Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51:1235–1243
     [Google Scholar]
  10. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. 2004; Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54:93–97 [CrossRef]
     [Google Scholar]
  11. Jit S., Dadhwal M., Prakash O., Lal R. 2008; Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 58:1665–1669 [CrossRef]
     [Google Scholar]
  12. Kim J. M., Le N. T., Chung B. S., Park J. H., Bae J.-W., Madsen E. L., Jeon C. O. 2008; Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o -, m -, and p -xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74:7313–7320 [CrossRef]
     [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  14. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  15. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  16. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. 2006; Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44:155–161
     [Google Scholar]
  17. McCammon S. A., Bowman J. P. 2000 Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063 [CrossRef]
  18. Park M., Lu S., Ryu S. H., Chung B. S., Park W., Kim C.-J., Jeon C. O. 2006; Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56:2443–2447 [CrossRef]
     [Google Scholar]
  19. Park M., Ryu S. H., Vu T. H., Ro H. S., Yun P. Y., Jeon C. O. 2007; Flavobacterium defluvii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 57:233–237 [CrossRef]
     [Google Scholar]
  20. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
     [Google Scholar]
  21. Ryu S. H., Park M., Jeon Y., Lee J. R., Park W., Jeon C. O. 2007; Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea. Int J Syst Evol Microbiol 57:2026–2030 [CrossRef]
     [Google Scholar]
  22. Ryu S. H., Park J. H., Moon J. C., Sung Y., Lee S. S., Jeon C. O. 2008; Flavobacterium resistens sp. nov., isolated from stream sediment. Int J Syst Evol Microbiol 58:2266–2270 [CrossRef]
     [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  24. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. 2003; Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53:519–526 [CrossRef]
     [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  27. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2005; Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55:769–772 [CrossRef]
     [Google Scholar]
  28. Vela A. I., Fernandez A., Sánchez-Porro C., Sierra E., Mendez M., Arbelo M., Ventosa A., Domínguez L., Fernández-Garayzábal J. F. 2007; Flavobacterium ceti sp. nov., isolated from beaked whales ( Ziphius cavirostris ). Int J Syst Evol Microbiol 57:2604–2608 [CrossRef]
     [Google Scholar]
  29. Weon H. Y., Song M. H., Son J. A., Kim B. Y., Kwon S. W., Go S. J., Stackebrandt E. 2007; Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 57:1594–1598 [CrossRef]
     [Google Scholar]
  30. Zhu F., Wang S., Zhou P. 2003; Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53:853–857 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010850-0
Loading
Flavobacterium fluvii sp. nov., isolated from stream sediment
Int J Syst Evol Microbiol 60, 353 (2010); https://doi.org/10.1099/ijs.0.010850-0
/content/journal/ijsem/10.1099/ijs.0.010850-0
/content/journal/ijsem/10.1099/ijs.0.010850-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error