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Abstract
A Gram-positive, aerobic and non-motile bacterial strain, designated ON-33T, was subjected to a study based on a polyphasic approach to determine its exact taxonomic position. Strain ON-33T grew optimally at pH 7.0–7.5 and 30 °C. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ON-33T fell within the clade comprising Gordonia species, clustering with Gordonia soli CC-AB07T, with which it exhibited 16S rRNA gene sequence similarity of 98.5 %. The chemotaxonomic properties of strain ON-33T were consistent with those shared by members of the genus Gordonia. The peptidoglycan type was based on meso-diaminopimelic acid and the whole-cell sugars were arabinose and galactose. The predominant menaquinone was MK-9(H2). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were C16 : 0, iso-C15 : 0 2-OH and/or C16 : 1 ω7c, 10-methyl C18 : 0 and C18 : 1 ω9c. The DNA G+C content was 66.9 mol%. Strain ON-33T exhibited a DNA–DNA relatedness value of 13 % to G. soli DSM 44995T and could be differentiated from G. soli by differences in phenotypic characteristics. On the basis of the data obtained, strain ON-33T is considered to represent a novel species of the genus Gordonia, for which the name Gordonia hankookensis sp. nov., is proposed. The type strain is ON-33T (=KCTC 19599T=CCUG 57507T).
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