Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste
Ueki, Atsuko and Abe, Kunihiro and Suzuki, Daisuke and Kaku, Nobuo and Watanabe, Kazuya and Ueki, Katsuji,, 59, 3161-3167 (2009),
doi = https://doi.org/10.1099/ijs.0.011858-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A strictly anaerobic, mesophilic and aminolytic strain (WN036T) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were Gram-positive cocci, often occurred in pairs and were non-motile. Although spore formation was not confirmed by microscopic observation of cells, the strain produced thermotolerant cells. The optimum temperature for growth was 35–37 °C and the optimum pH was 6.7. Oxidase, catalase and nitrate-reducing activities were negative. The novel strain did not ferment carbohydrates and grew in PY medium without additional substrates. The strain utilized l-glutamate, l-glutamine, l-histidine and l-arginine as growth substrates. Major fermentation products were acetate and butyrate with a small amount of propionate. The genomic DNA G+C content was 32.5 mol%. The major cellular fatty acids were C17 : 1 ω8, C18 : 1 ω7 DMA and C16 : 0. The diagnostic diamino acid of the cell-wall peptidoglycan was lysine. Glutamic acid, glycine, alanine and aspartic acid were also detected in the cell-wall peptidoglycan. On the basis of 16S rRNA gene sequences, the most closely related species to strain WN036T were Peptoniphilus asaccharolyticus ATCC 14965T (89.8 %) and Peptoniphilus indolicus ATCC 29427T (89.6 %). Based on the differences in the phenotypic and phylogenetic characteristics of strain WN036T compared with those of closely related species, a novel genus and species, Anaerosphaera aminiphila gen. nov., sp. nov., is proposed. The type strain is WN036T (=JCM 15094T=DSM 21120T).,
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